Monomeric prefusion structure of an extremophile gamete fusogen and stepwise formation of the postfusion trimeric state.
Monomeric prefusion structure of an extremophile gamete fusogen and stepwise formation of the postfusion trimeric state.
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DOI：
10.1038/s41467-022-31744-z
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发表时间：
2022-07-13
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
在这里，我们研究了一种生长在酸性pH值为45°C的嗜极红藻Cyanidioschyzon merolae （Cyani）的配子fusogen HAP2。HAP2具有与病毒II类融合蛋白相似的三聚体融合后结构，但其融合前结构尚不清楚。Cyani HAP2单体预熔态的晶体结构显示其具有高度扩展的D2、D1和D3三个结构域。在D2顶端的三个疏水融合环是融合后状态形成所必需的。我们在洗涤剂胶束刺激后融合状态形成的过程中进行了负染色电镜步骤。在中间状态下，两个或三个线性HAP2单体结合在D2末端，形成其融合环。随后，D2和D1排列在三聚体的核心，D3折叠在D1和D2的外部。中间或融合后样状态的形成不需要D3。对保守的配子融合原HAP2的融合后结构的研究显示了病毒II类融合蛋白典型的三聚体状态。在这里，作者展示了Cyanidioschyzon merolae HAP2预融合状态的晶体结构和通往其三聚体融合后状态的中间体的低分辨率结构。
Here, we study the gamete fusogen HAP2 from Cyanidioschyzon merolae (Cyani), an extremophile red algae that grows at acidic pH at 45 °C. HAP2 has a trimeric postfusion structure with similarity to viral class II fusion proteins, but its prefusion structure has been elusive. The crystal structure of a monomeric prefusion state of Cyani HAP2 shows it is highly extended with three domains in the order D2, D1, and D3. Three hydrophobic fusion loops at the tip of D2 are each required for postfusion state formation. We followed by negative stain electron microscopy steps in the process of detergent micelle-stimulated postfusion state formation. In an intermediate state, two or three linear HAP2 monomers associate at the end of D2 bearing its fusion loops. Subsequently, D2 and D1 line the core of a trimer and D3 folds back over the exterior of D1 and D2. D3 is not required for formation of intermediate or postfusion-like states. Studies in the postfusion structures of the conserved gamete fusogen HAP2 show a trimeric state typical of viral class II fusion proteins. Here, the authors present the crystal structure of a prefusion state of Cyanidioschyzon merolae HAP2 and low resolution structures of intermediates in the pathway to its trimeric postfusion state.
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影响因子：
9.8
作者：
Clark T
通讯作者：
Clark T
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
2.9
作者：
Tumolo, T;Angnes, L;Baptista, MS
通讯作者：
Baptista, MS
影响因子：
7.7
作者：
Feng, Juan;Dong, Xianchi;Springer, Timothy A.
通讯作者：
Springer, Timothy A.