High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis.
High-resolution transcriptional analysis of the regulatory influence of cell-to-cell signalling reveals novel genes that contribute to Xanthomonas phytopathogenesis.
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DOI：
10.1111/mmi.12229
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发表时间：
2013-06
影响因子：
3.6
通讯作者：
Ryan RP
中科院分区：
文献类型：
--
作者：
An SQ;Febrer M;McCarthy Y;Tang DJ;Clissold L;Kaithakottil G;Swarbreck D;Tang JL;Rogers J;Dow JM;Ryan RP
野油菜黄单胞菌（Xanthomonascampestris）是一种重要的经济病原菌，也是细菌性植物病原学研究的模式菌。在X。油菜毒力是由DSF信号分子介导的调控系统控制的。DSF信号的合成和识别取决于不同的Rpf蛋白。DSF信号产生需要RpfF，而信号感知和转导取决于包括传感器RpfC和调节器RpfG的系统。在这里，我们已经解决了行动和作用的Rpf/DSF信号在植物病原体的高分辨率转录分析耦合功能基因组学。我们检测到许多基因的转录本，这些基因是以前对基因组序列的计算分析所识别的。新的转录区域包括预测为编码或非编码的基因间转录物以及与编码序列反义的基因间转录物。总的来说，rpfF、rpfG和rpfC的突变导致约480个基因的转录水平改变（超过四倍）。RpfF和RpfC的调节影响表现出相当大的重叠。与预期相反，RpfC和RpfG的调节影响有有限的重叠，表明Rpf信号系统的复杂性。重要的是，功能分析揭示了Rpf调控基因组中超过160个新的毒力因子。
The bacterium Xanthomonas campestris is an economically important pathogen of many crop species and a model for the study of bacterial phytopathogenesis. In X. campestris, a regulatory system mediated by the signal molecule DSF controls virulence to plants. The synthesis and recognition of the DSF signal depends upon different Rpf proteins. DSF signal generation requires RpfF whereas signal perception and transduction depends upon a system comprising the sensor RpfC and regulator RpfG. Here we have addressed the action and role of Rpf/DSF signalling in phytopathogenesis by high-resolution transcriptional analysis coupled to functional genomics. We detected transcripts for many genes that were unidentified by previous computational analysis of the genome sequence. Novel transcribed regions included intergenic transcripts predicted as coding or non-coding as well as those that were antisense to coding sequences. In total, mutation of rpfF, rpfG and rpfC led to alteration in transcript levels (more than fourfold) of approximately 480 genes. The regulatory influence of RpfF and RpfC demonstrated considerable overlap. Contrary to expectation, the regulatory influence of RpfC and RpfG had limited overlap, indicating complexities of the Rpf signalling system. Importantly, functional analysis revealed over 160 new virulence factors within the group of Rpf-regulated genes.
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影响因子：
3.7
作者：
Beaume M;Hernandez D;Farinelli L;Deluen C;Linder P;Gaspin C;Romby P;Schrenzel J;Francois P
通讯作者：
Francois P
影响因子：
14.9
作者：
Mandin P;Repoila F;Vergassola M;Geissmann T;Cossart P
通讯作者：
Cossart P
DOI：
10.1073/pnas.0912839107
发表时间：
2010-03-30
影响因子：
11.1
作者：
Ryan, Robert P.;McCarthy, Yvonne;Dow, J. Maxwell
通讯作者：
Dow, J. Maxwell
影响因子：
7
作者：
Qian, W;Jia, YT;He, CZ
通讯作者：
He, CZ
影响因子：
3.6
作者：
Ryan, Robert P.;Fouhy, Yvonne;Dow, J. Maxwell
通讯作者：
Dow, J. Maxwell