Cartography of methicillin-resistant S. aureus transcripts: detection, orientation and temporal expression during growth phase and stress conditions.
Cartography of methicillin-resistant S. aureus transcripts: detection, orientation and temporal expression during growth phase and stress conditions.
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DOI：
10.1371/journal.pone.0010725
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发表时间：
2010-05-20
期刊：
影响因子：
3.7
通讯作者：
Francois P
中科院分区：
文献类型：
--
作者：
Beaume M;Hernandez D;Farinelli L;Deluen C;Linder P;Gaspin C;Romby P;Schrenzel J;Francois P
金黄色葡萄球菌是一种多才多艺的细菌机会主义者，可引起广泛的感染。这些感染的严重程度是高度可变的，取决于多种参数，包括细菌的基因组含量以及受感染宿主的状况。临床和流行病学上，金黄色葡萄球菌显示出一种特殊的生存能力和适应剧烈环境变化的能力，包括许多抗菌剂的存在。尽管进行了重要的研究，但引发这种适应的机制在很大程度上仍然未知。迄今为止，大多数评估基因含量的研究都忽略了对所谓的基因间区以及潜在的反义RNA分子的分析。利用高通量测序技术，我们对金黄色葡萄球菌菌株N315的整个转录组进行了盘点。在金黄色葡萄球菌菌株N315的染色体和质粒中，除了注释的转录单位外，我们还鉴定了超过195个小转录区。每个转录本的编码链被识别，结构分析使所有发现的转录本分类。在细菌生长阶段的四个时间点纯化的RNA使我们能够定义这些转录本的时间表达。在不同的胁迫条件下，包括pH值、温度、氧化冲击和在严格的培养基中生长，我们评估了分布在基因间区域的26个感兴趣的转录本的表达变化。这些转录本中的大多数都显示了我们测试的特定压力条件下的表达模式。这些RNA分子可能代表金黄色葡萄球菌适应的重要效应物，并且更普遍地支持细菌的一些流行病学特征。
Staphylococcus aureus is a versatile bacterial opportunist responsible for a wide spectrum of infections. The severity of these infections is highly variable and depends on multiple parameters including the genome content of the bacterium as well as the condition of the infected host. Clinically and epidemiologically, S. aureus shows a particular capacity to survive and adapt to drastic environmental changes including the presence of numerous antimicrobial agents. Mechanisms triggering this adaptation remain largely unknown despite important research efforts. Most studies evaluating gene content have so far neglected to analyze the so-called intergenic regions as well as potential antisense RNA molecules. Using high-throughput sequencing technology, we performed an inventory of the whole transcriptome of S. aureus strain N315. In addition to the annotated transcription units, we identified more than 195 small transcribed regions, in the chromosome and the plasmid of S. aureus strain N315. The coding strand of each transcript was identified and structural analysis enabled classification of all discovered transcripts. RNA purified at four time-points during the growth phase of the bacterium allowed us to define the temporal expression of such transcripts. A selection of 26 transcripts of interest dispersed along the intergenic regions was assessed for expression changes in the presence of various stress conditions including pH, temperature, oxidative shocks and growth in a stringent medium. Most of these transcripts showed expression patterns specific for the defined stress conditions that we tested. These RNA molecules potentially represent important effectors of S. aureus adaptation and more generally could support some of the epidemiological characteristics of the bacterium.
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影响因子：
4.8
作者：
Chien, YT;Manna, AC;Cheung, AL
通讯作者：
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