Astrocyte and Neuronal Panx1 Support Long-Term Reference Memory in Mice.
Astrocyte and Neuronal Panx1 Support Long-Term Reference Memory in Mice.
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DOI：
10.1177/17590914231184712
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发表时间：
2023-01
期刊：
影响因子：
4.7
通讯作者：
Scemes, Eliana
中科院分区：
文献类型：
--
作者：
Obot, Price;Subah, Galadu;Schonwald, Antonia;Pan, Jian;Velisek, Libor;Veliskova, Jana;Stanton, Patric K.;Scemes, Eliana
Pannexin 1 (Panx1) 是一种普遍表达的蛋白质，可形成可渗透阴离子和中等大小信号分子（例如 ATP、谷氨酸）的质膜通道。在神经系统中，Panx1 通道的激活已被广泛证明会导致不同的神经系统疾病（癫痫、慢性疼痛、偏头痛、神经艾滋病等），但对这些通道的生理作用程度的了解仍然仅限于支持其参与海马依赖性学习的三项研究。鉴于 Panx1 通道可能为活动依赖性神经元-胶质细胞相互作用提供重要机制，我们使用具有全局和细胞类型特异性 Panx1 缺失的 Panx1 转基因小鼠来询问它们对工作记忆和参考记忆的参与。使用八臂径向迷宫，我们发现 Panx1 缺失小鼠缺乏长期空间参考记忆，但不存在空间工作记忆，并且星形胶质细胞和神经元 Panx1 都有助于巩固长期空间记忆。 Panx1缺失小鼠海马切片的场电位记录显示，Schaffer侧支CA1突触的突触强度长期增强（LTP）和长期抑制（LTD）均减弱，但基础突触传递或突触前配对脉冲促进没有改变。我们的结果表明神经元和星形胶质细胞 Panx1 通道是小鼠长期空间参考记忆的发展和维持的关键参与者。
Pannexin 1 (Panx1) is an ubiquitously expressed protein that forms plasma membrane channels permeable to anions and moderate-sized signaling molecules (e.g., ATP, glutamate). In the nervous system, activation of Panx1 channels has been extensively shown to contribute to distinct neurological disorders (epilepsy, chronic pain, migraine, neuroAIDS, etc.), but knowledge of the extent to which these channels have a physiological role remains restricted to three studies supporting their involvement in hippocampus dependent learning. Given that Panx1 channels may provide an important mechanism for activity-dependent neuron-glia interaction, we used Panx1 transgenic mice with global and cell-type specific deletions of Panx1 to interrogate their participation in working and reference memory. Using the eight-arm radial maze, we show that long-term spatial reference memory, but not spatial working memory, is deficient in Panx1-null mice and that both astrocyte and neuronal Panx1 contribute to the consolidation of long-term spatial memory. Field potential recordings in hippocampal slices of Panx1-null mice revealed an attenuation of both long-term potentiation (LTP) of synaptic strength and long-term depression (LTD) at Schaffer collateral-CA1 synapses without alterations of basal synaptic transmission or pre-synaptic paired-pulse facilitation. Our results implicate both neuronal and astrocyte Panx1 channels as critical players for the development and maintenance of long-term spatial reference memory in mice.
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影响因子：
3.3
作者：
Garcia-Alvarez G;Lu B;Yap KA;Wong LC;Thevathasan JV;Lim L;Ji F;Tan KW;Mancuso JJ;Tang W;Poon SY;Augustine GJ;Fivaz M
通讯作者：
Fivaz M
DOI：
10.1073/pnas.1008200107
发表时间：
2010-09-21
影响因子：
11.1
作者：
Ge, Yuan;Dong, Zhifang;Wang, Yu Tian
通讯作者：
Wang, Yu Tian
DOI：
10.1073/pnas.2233464100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bruzzone, R;Hormuzdi, SG;Monyer, H
通讯作者：
Monyer, H
影响因子：
56.9
作者：
Brun, VH;Otnæss, MK;Moser, EI
通讯作者：
Moser, EI
影响因子：
20.1
作者：
Billaud M;Lohman AW;Straub AC;Looft-Wilson R;Johnstone SR;Araj CA;Best AK;Chekeni FB;Ravichandran KS;Penuela S;Laird DW;Isakson BE
通讯作者：
Isakson BE