Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates.
Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to fine particulates.
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尿液8-羟基-2'-脱氧鸟苷是暴露于细颗粒的工人中氧化DNA损伤的生物标志物。
DOI：
10.1289/ehp.6827
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发表时间：
2004-05
影响因子：
10.4
通讯作者：
Christiani DC
中科院分区：
文献类型：
--
作者：
Kim JY;Mukherjee S;Ngo LC;Christiani DC
残留油飞灰(ROFA)是一种化学上复杂的化合物混合物，包括可能致癌的金属，因为它们能够引起氧化损伤。在这项研究中，我们调查了暴露于空气动力学质量中值直径<；或=2.5微米(PM2.5)的颗粒物与DNA氧化损伤和修复之间的关系，如尿8-羟基-2‘-脱氧鸟苷(8-OHdG)浓度所表明的那样，暴露在ROFA和金属烟雾中的一组锅炉制造商中。在燃油锅炉大修期间，对20名工人(50%吸烟者)进行了为期5天的监测。职业PM2.5 8小时时间加权平均值的中位数为0.44毫克/立方米(第25-75百分位数，0.29-0.76)。经+/-SE肌酸调节的8-OHdG平均水平，班前尿样为13.26+/-1.04微克/克，班后尿样为15.22+/-0.99微克/克。在调整尿可替宁水平、慢性支气管炎状况和年龄后，轮班后样本的尿8-OHdG水平显著高于轮班前样本(p=0.02)。线性混合模型显示PM2.5暴露与尿8-OHdG水平之间存在显著的暴露-反应关联(p=0.03)。PM2.5暴露每增加1毫克/立方米，8-OHdG水平就增加1.67微克/克(95%可信区间，0.21-3.14)。接触PM2.5中的钒、锰、镍和铅也与8-OHdG水平呈正相关(P<；OR=0.05)。这项研究表明，相对年轻和健康的一群锅炉制造商在暴露于高水平的含金属颗粒物后，可能会经历发生氧化DNA损伤的风险增加。
Residual oil fly ash (ROFA) is a chemically complex mixture of compounds, including metals that are potentially carcinogenic because of their ability to cause oxidative injury. In this study, we investigated the association between exposure to particulate matter with an aerodynamic mass median diameter <or= 2.5 micro m (PM2.5) and oxidative DNA damage and repair, as indicated by urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, in a group of boilermakers exposed to ROFA and metal fumes. Twenty workers (50% smokers) were monitored for 5 days during an overhaul of oil-fired boilers. The median occupational PM2.5 8-hr time-weighted average was 0.44 mg/m3 (25th-75th percentile, 0.29-0.76). The mean +/- SE creatinine-adjusted 8-OHdG levels were 13.26 +/- 1.04 micro g/g in urine samples collected pre-workshift and 15.22 +/- 0.99 micro g/g in the post-workshift samples. The urinary 8-OHdG levels were significantly greater in the post-workshift samples than in the pre-workshift samples (p = 0.02), after adjusting for urinary cotinine levels, chronic bronchitis status, and age. Linear mixed models indicated a significant exposure-response association between PM2.5 exposure and urinary 8-OHdG levels (p = 0.03). Each 1-mg/m3 incremental increase in PM2.5 exposure was associated with an increase of 1.67 micro g/g (95% confidence interval, 0.21-3.14) in 8-OHdG levels. PM2.5 vanadium, manganese, nickel, and lead exposures also were positively associated with 8-OHdG levels (p <or= 0.05). This study suggests that a relatively young and healthy cohort of boilermakers may experience an increased risk of developing oxidative DNA injury after exposure to high levels of metal-containing particulate matter.
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影响因子：
4.7
作者：
KASAI, H;CRAIN, PF;TANOOKA, H
通讯作者：
TANOOKA, H
影响因子：
1.8
作者：
Hartwig, A
通讯作者：
Hartwig, A
影响因子：
2.7
作者：
Honda, M;Yamada, Y;Kamihira, S
通讯作者：
Kamihira, S
DOI：
10.1007/s004200050276
发表时间：
1998-06-01
影响因子：
3
作者：
Anttila, A;Pukkala, E;Karjalainen, S
通讯作者：
Karjalainen, S
影响因子：
6.3
作者：
COOPER, WC;WONG, O;KHEIFETS, L
通讯作者：
KHEIFETS, L