Functional Redundancy of FLOWERING LOCUS T 3b in Soybean Flowering Time Regulation.
Functional Redundancy of FLOWERING LOCUS T 3b in Soybean Flowering Time Regulation.
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DOI：
10.3390/ijms23052497
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发表时间：
2022-02-24
影响因子：
5.6
通讯作者：
Hou W
中科院分区：
文献类型：
--
作者：
Su Q;Chen L;Cai Y;Chen Y;Yuan S;Li M;Zhang J;Sun S;Han T;Hou W
光周期开花是决定大豆适应性和产量的重要农艺性状，受开花位点T（开花位点T）基因的强烈影响。由于基因组中存在多个FT同源物，它们在大豆中的功能尚不完全清楚。在这里，我们发现GmFT3b在调节大豆光周期开花中表现出功能冗余。生物信息学分析表明，GmFT3b是典型的花诱导剂FT同源物，该蛋白定位于细胞核。此外，GmFT3b的表达受光周期和昼夜节律的诱导，并且对长日（LD）条件更敏感。我们获得了一个纯合子敲除ft3b和三个过表达gmft3b的大豆系，用于不同光周期下的评估。在自然长日照、短日照或LD条件下，野生型、GmFT3b过表达者和ft3b敲除者的开花时间没有显著差异。虽然下游开花相关基因GmFUL1 （a, b）、GmAP1d和GmLFY1在ft3b植物中略有下调，但开花诱导因子GmFT5a和GmFT5b却高表达，表明GmFT3b的缺失可能得到补偿。我们认为GmFT3b在大豆开花时间调控中起冗余作用，可能被其他FT同源物补偿。
Photoperiodic flowering is an important agronomic trait that determines adaptability and yield in soybean and is strongly influenced by FLOWERING LOCUS T (FT) genes. Due to the presence of multiple FT homologs in the genome, their functions in soybean are not fully understood. Here, we show that GmFT3b exhibits functional redundancy in regulating soybean photoperiodic flowering. Bioinformatic analysis revealed that GmFT3b is a typical floral inducer FT homolog and that the protein is localized to the nucleus. Moreover, GmFT3b expression was induced by photoperiod and circadian rhythm and was more responsive to long-day (LD) conditions. We generated a homozygous ft3b knockout and three GmFT3b-overexpressing soybean lines for evaluation under different photoperiods. There were no significant differences in flowering time between the wild-type, the GmFT3b overexpressors, and the ft3b knockouts under natural long-day, short-day, or LD conditions. Although the downstream flowering-related genes GmFUL1 (a, b), GmAP1d, and GmLFY1 were slightly down-regulated in ft3b plants, the floral inducers GmFT5a and GmFT5b were highly expressed, indicating potential compensation for the loss of GmFT3b. We suggest that GmFT3b acts redundantly in flowering time regulation and may be compensated by other FT homologs in soybean.
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DOI：
10.1016/j.cub.2010.01.059
发表时间：
2010-04-13
期刊：
Current biology : CB
影响因子：
--
作者：
Blackman BK;Strasburg JL;Raduski AR;Michaels SD;Rieseberg LH
通讯作者：
Rieseberg LH
影响因子：
2.6
作者：
HYMOWITZ, T
通讯作者：
HYMOWITZ, T
影响因子：
7.3
作者：
Chen, Li;Cai, Yupeng;Hou, Wensheng
通讯作者：
Hou, Wensheng
影响因子：
2.1
作者：
Carpentieri-Pípolo, Valéria;Almeida, Leones Alves de;Kiihl, Romeu Afonso de S.
通讯作者：
Kiihl, Romeu Afonso de S.
影响因子：
5.6
作者：
Cai, Yupeng;Chen, Li;Hou, Wensheng
通讯作者：
Hou, Wensheng