The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana.
The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana.
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DOI：
10.3390/ijms22115856
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发表时间：
2021-05-29
影响因子：
5.6
通讯作者：
Men S
中科院分区：
文献类型：
--
作者：
Arif M;Li Z;Luo Q;Li L;Shen Y;Men S
BAG蛋白是一个多功能共分子伴侣家族。在植物中，BAG蛋白被发现在非生物和生物胁迫耐受中发挥作用。然而，拟南芥BAG2的功能在很大程度上仍然未知，而BAG6是植物防御病原体所必需的，尽管BAG6是否参与植物对非生物胁迫的耐受性仍然未知。在这里，我们表明，BAG2和BAG6在各种组织中表达，并上调盐，甘露醇，热处理和应激相关激素，包括阿坝，乙烯和SA。与野生型（WT）相比，bag2、bag6和bag2bag6种子的萌发对阿坝不太敏感，而BAG2和BAG6过表达系对阿坝超敏感。bag2、bag6和bag2bag6植物在干旱处理中显示出比WT更高的存活率，但在热胁迫处理中显示出更低的存活率。一致地，这些突变体与WT相比显示出若干胁迫和ABA相关基因如RD29A、RD29B、NCED3和ABI 4的差异表达。此外，这些突变体在干旱和阿坝处理后表现出较低的ROS水平，但在热处理后比WT表现出较高的ROS积累。这些结果表明，BAG2和BAG6在拟南芥中与干旱胁迫负相关，但在热胁迫中起积极作用。
The BAG proteins are a family of multi-functional co-chaperones. In plants, BAG proteins were found to play roles both in abiotic and biotic stress tolerance. However, the function of Arabidopsis BAG2 remains largely unknown, whereas BAG6 is required for plants’ defense to pathogens, although it remains unknown whether BAG6 is involved in plants’ tolerance to abiotic stresses. Here, we show that both BAG2 and BAG6 are expressed in various tissues and are upregulated by salt, mannitol, and heat treatments and by stress-related hormones including ABA, ethylene, and SA. Germination of bag2, bag6 and bag2 bag6 seeds is less sensitive to ABA compared to the wild type (WT), whereas BAG2 and BAG6 overexpression lines are hypersensitive to ABA. bag2, bag6, and bag2 bag6 plants show higher survival rates than WT in drought treatment but display lower survival rates in heat-stress treatment. Consistently, these mutants showed differential expression of several stress- and ABA-related genes such as RD29A, RD29B, NCED3 and ABI4 compared to the WT. Furthermore, these mutants exhibit lower levels of ROS after drought and ABA treatment but higher ROS accumulation after heat treatment than the WT. These results suggest that BAG2 and BAG6 are negatively involved in drought stress but play a positive role in heat stress in Arabidopsis.
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影响因子：
5.2
作者：
Jain S;Wiemann P;Thill E;Williams B;Keller NP;Kabbage M
通讯作者：
Kabbage M
影响因子：
6.1
作者：
Kapadia M;De Snoo ML;Kalia LV;Kalia SK
通讯作者：
Kalia SK
影响因子：
2.9
作者：
Nawkar, Ganesh M.;Maibam, Punyakishore;Park, Joung Hun;Woo, Su Gyeong;Kim, Cha Young;Lee, Sang Yeol;Kang, Chang Ho
通讯作者：
Kang, Chang Ho
DOI：
10.1006/bbrc.2001.5512
发表时间：
2001-09-07
影响因子：
3.1
作者：
Antoku, K;Maser, RS;Johnson, DE
通讯作者：
Johnson, DE
影响因子：
5.1
作者：
Nakashima, K;Fujita, Y;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K