LINC00536 Promotes Breast Cancer Progression by Regulating ROCK1 via Sponging of miR-214-5p
LINC00536 Promotes Breast Cancer Progression by Regulating ROCK1 via Sponging of miR-214-5p
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LINC00536 通过 miR-214-5p 海绵作用调节 ROCK1 促进乳腺癌进展
DOI：
10.1007/s10528-022-10304-6
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发表时间：
2022-12
影响因子：
2.4
通讯作者：
Lihua Li
中科院分区：
文献类型：
--
作者：
Caixia Hu;Xiufen Zhang;Kai Fang;Zijian Guo;Lihua Li
Accumulating evidence has shown that long noncoding RNAs (lncRNAs) play a significant role in regulating gene expression and participating in the progression of various malignancies. In our study, by analyzing data from The Cancer Genome Atlas (TCGA), LINC00536 was found to be highly expressed in breast cancer (BC) tissues, but its function and clinical significance in BC are still unknown. Therefore, we aimed to explore the role and molecular mechanism of LINC00536 in BC. We collected human BC tissue specimens and validated that LINC00536 was overexpressed in BC tissues. Increased LINC00536 expression was associated with advanced TNM stage, larger tumor diameter, lymph node metastasis and poor prognosis in patients with BC. Univariate and multivariate Cox regression analyses showed that high LINC00536 expression was an independent prognostic risk factor for overall survival in BC patients. Furthermore, quantitative reverse transcription PCR (qRT-PCR) showed that LINC00536 was upregulated in BC cell lines. Then, we confirmed that LINC00536 silencing-inhibited BC cell proliferation, migration, and invasion and led to cell cycle arrest in vitro. Animal experiments showed that knockdown of LINC00536 expression suppressed tumorigenesis in vivo. Mechanistically, LINC00536 serves as a ceRNA for miR-214-5p, increasing the expression of ROCK1, which acts as a tumor promoter in BC. Rescue assays revealed that miR-214-5p inhibition or ROCK1 overexpression could neutralize the suppressive effects of LINC00536 knockdown on cell proliferation, migration and invasion. Our data indicated that LINC00536 accelerates BC progression by regulating the miR-214-5p/ROCK1 pathway, which might provide a new perspective to investigate the development process of BC.
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DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
10.3
作者：
Borin TF;Arbab AS;Gelaleti GB;Ferreira LC;Moschetta MG;Jardim-Perassi BV;Iskander AS;Varma NR;Shankar A;Coimbra VB;Fabri VA;de Oliveira JG;Zuccari DA
通讯作者：
Zuccari DA
影响因子：
8
作者：
Peng WX;Koirala P;Mo YY
通讯作者：
Mo YY
影响因子：
3.1
作者：
Zhang M;Wang D;Zhu T;Yin R
通讯作者：
Yin R
影响因子：
4.8
作者：
Zhuang, Chengle;Ma, Qian;Gui, Yaoting
通讯作者：
Gui, Yaoting