Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping.
Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping.
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DOI：
10.1371/journal.pone.0007465
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发表时间：
2009-10-15
期刊：
影响因子：
3.7
通讯作者：
Sahara K
中科院分区：
文献类型：
--
作者：
Yasukochi Y;Tanaka-Okuyama M;Shibata F;Yoshido A;Marec F;Wu C;Zhang H;Goldsmith MR;Sahara K
基因组测序项目已经完成了几个物种代表四个高度分歧的全变态昆虫目，双翅目，Hyplutera，鞘翅目，鳞翅目。昆虫惊人的进化多样性表明，需要有效的方法来将这些模型的基因组信息应用于遗传上没有特征的物种。构建保守的同线性图在这项任务中起着至关重要的作用。在这里，我们展示了使用荧光原位杂交与细菌人工染色体探针作为一个强大的工具，物理映射的基因和比较基因组分析在鳞翅目，其中有众多的和形态均匀的全动染色体。我们分离了214个克隆，其中包含159个已测序的鳞翅目模型（家蚕）的保守单拷贝基因的直系同源物，这些基因来自具有未探索基因组的鞘氨醇类（烟草天蛾）的BAC文库。然后，我们构建了一个BAC-FISH核型识别所有28条染色体的M。使用相应的BAC克隆对124个基因座进行定位，从而获得sexta。三个M. sexta染色体也在旋花天蛾的相应染色体上产生了清晰的信号，旋花天蛾与天蛾属于同一亚科。六。M. Sexta BAC物理图谱与B的连锁图谱和基因组序列。Mori指出，在大多数染色体中广泛的保守共线性包括保守的基因顺序。只有少数重排，包括三个倒位，三个易位，和两个裂变/融合事件估计已经发生后的分歧，天蛾科和天蛾科。这些结果为鳞翅目昆虫基因组的稳定性提供了越来越多的证据。在A上生成信号。旋花染色体，利用异源M. sexta探针表明，BAC-FISH与orthopathic序列可用于核型分析的范围广泛的相关和遗传上的uncharacterized物种，显着扩展的能力，发展同线性地图的比较和功能基因组学。
Genome sequencing projects have been completed for several species representing four highly diverged holometabolous insect orders, Diptera, Hymenoptera, Coleoptera, and Lepidoptera. The striking evolutionary diversity of insects argues a need for efficient methods to apply genome information from such models to genetically uncharacterized species. Constructing conserved synteny maps plays a crucial role in this task. Here, we demonstrate the use of fluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes. We isolated 214 clones containing 159 orthologs of well conserved single-copy genes of a sequenced lepidopteran model, the silkworm, Bombyx mori, from a BAC library of a sphingid with an unexplored genome, the tobacco hornworm, Manduca sexta. We then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124 loci using the corresponding BAC clones. BAC probes from three M. sexta chromosomes also generated clear signals on the corresponding chromosomes of the convolvulus hawk moth, Agrius convolvuli, which belongs to the same subfamily, Sphinginae, as M. sexta. Comparison of the M. sexta BAC physical map with the linkage map and genome sequence of B. mori pointed to extensive conserved synteny including conserved gene order in most chromosomes. Only a few rearrangements, including three inversions, three translocations, and two fission/fusion events were estimated to have occurred after the divergence of Bombycidae and Sphingidae. These results add to accumulating evidence for the stability of lepidopteran genomes. Generating signals on A. convolvuli chromosomes using heterologous M. sexta probes demonstrated that BAC-FISH with orthologous sequences can be used for karyotyping a wide range of related and genetically uncharacterized species, significantly extending the ability to develop synteny maps for comparative and functional genomics.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
3.7
作者：
Kawahara AY;Mignault AA;Regier JC;Kitching IJ;Mitter C
通讯作者：
Mitter C
DOI：
10.1007/1-4020-4051-2_5
发表时间：
2005-01-01
期刊：
STERILE INSECT TECHNIQUE: PRINCIPLES AND PRACTICE IN AREA-WIDE INTEGRATED PEST MANAGEMENT
影响因子：
--
作者：
Carpenter, J. E.;Bloem, S.;Marec, F.
通讯作者：
Marec, F.
影响因子：
11.6
作者：
Nagaki, K;Kashihara, K;Murata, M
通讯作者：
Murata, M
影响因子：
2.9
作者：
Eleftherianos, Ioannis;Millichap, Peter J.;Reynolds, Stuart E.
通讯作者：
Reynolds, Stuart E.