SOX2 regulates homeostasis of taste bud cells and lingual epithelial cells in posterior tongue.
SOX2 regulates homeostasis of taste bud cells and lingual epithelial cells in posterior tongue.
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Sox2调节后舌的味蕾细胞和舌上皮细胞的稳态。
DOI：
10.1371/journal.pone.0240848
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Matsumoto I
中科院分区：
文献类型：
--
作者：
Ohmoto M;Lei W;Yamashita J;Hirota J;Jiang P;Matsumoto I
味蕾细胞起源于口腔中的局部上皮干细胞，并且在动物的一生中不断被新生细胞取代。然而，很少有人知道的分子和细胞机制的味觉细胞营业额。最近，已经证明，SOX 2，一种在口腔的上皮干/祖细胞中表达的转录因子，调节包括味觉和非味觉乳头的前舌上皮的更新。然而，SOX 2在调节舌后部味觉细胞更新中的作用尚不清楚。由于舌前部和后部的味蕾和干/祖细胞的细胞和分子组成存在区域差异，这些细胞和细胞来源于不同的胚胎来源，我们着手确定SOX 2在舌后部上皮组织稳态中的作用。在这里，我们报告的差异要求SOX 2的干/祖细胞的舌上皮细胞在舌后正常营业额。Sox 2在干/祖细胞中的缺失既不诱导活性半胱天冬酶3介导的凋亡性细胞死亡，也不改变舌后部的干/祖细胞群。然而，非味觉上皮细胞的形态和分子特征在轮廓乳头中受损，而在丝状乳头中没有。值得注意的是，随着时间的推移，味蕾变得更薄，崩溃，无法检测到。Sox 2缺失的干/祖细胞的谱系追踪表明味蕾中几乎完全缺乏新产生的基底前体细胞，这表明Sox 2参与了决定干/祖细胞分化为味觉谱系细胞的机制。总之，这些结果表明，SOX 2在调节舌后部上皮组织稳态中起关键作用，与其在舌前部的功能相似但不相同。
Taste bud cells arise from local epithelial stem cells in the oral cavity and are continuously replaced by newborn cells throughout an animal’s life. However, little is known about the molecular and cellular mechanisms of taste cell turnover. Recently, it has been demonstrated that SOX2, a transcription factor expressed in epithelial stem/progenitor cells of the oral cavity, regulates turnover of anterior tongue epithelium including gustatory and non-gustatory papillae. Yet, the role of SOX2 in regulating taste cell turnover in the posterior tongue is unclear. Prompted by the fact that there are regional differences in the cellular and molecular composition of taste buds and stem/progenitor cells in the anterior and posterior portions of tongue, which are derived from distinct embryonic origins, we set out to determine the role of SOX2 in epithelial tissue homeostasis in the posterior tongue. Here we report the differential requirement of SOX2 in the stem/progenitor cells for the normal turnover of lingual epithelial cells in the posterior tongue. Sox2 deletion in the stem/progenitor cells neither induced active caspase 3-mediated apoptotic cell death nor altered stem/progenitor cell population in the posterior tongue. Nevertheless, morphology and molecular feature of non-gustatory epithelial cells were impaired in the circumvallate papilla but not in the filiform papillae. Remarkably, taste buds became thinner, collapsed, and undetectable over time. Lineage tracing of Sox2-deleted stem/progenitor cells demonstrated an almost complete lack of newly generated basal precursor cells in the taste buds, suggesting mechanistically that Sox2 is involved in determining stem/progenitor cells to differentiate to gustatory lineage cells. Together, these results demonstrate that SOX2 plays key roles in regulating epithelial tissue homeostasis in the posterior tongue, similar but not identical to its function in the anterior tongue.
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影响因子：
25
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0607399104
发表时间：
2007-02-13
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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