Functional analysis of kinetochore assembly in Caenorhabditis elegans.
Functional analysis of kinetochore assembly in Caenorhabditis elegans.
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DOI：
10.1083/jcb.153.6.1209
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发表时间：
2001-06-11
期刊：
影响因子：
--
通讯作者：
Hyman AA
中科院分区：
文献类型：
--
作者：
Oegema K;Desai A;Rybina S;Kirkham M;Hyman AA
在所有真核生物中，有丝分裂染色体的分离需要它们与纺锤体微管的相互作用。为了剖析这种相互作用，我们使用活的和固定的测定在单细胞阶段秀丽隐杆线虫胚胎。我们比较了着丝粒组蛋白CENP-A，动粒结构组分CENP-C和染色体乘客蛋白INCENP的耗尽同系物的后果。CeCENP-A或CeCENP-C的耗尽导致相同的“动粒无效”表型，其特征在于有丝分裂染色体分离的完全失败以及招募其他动粒组分和组装机械稳定的纺锤体的失败。它们的耗尽表型的相似性，再加上CeCENP-A需要定位CeCENP-C，但反之亦然，表明动粒组装的关键步骤是含CENP-A的染色质招募CENP-C。CeINCENP耗尽胚胎的平行分析显示，有丝分裂染色体分离缺陷不同于CeCENP-A/C缺失时观察到的缺陷。在分裂后期之前和期间观察到缺陷，但染色质分离成两个大小相等的团块。机械稳定的纺锤体组装，在后期和末期显示出缺陷。此外，动粒组装和CeINCENP向染色体的募集是独立的。这些结果表明动粒和染色体乘客在有丝分裂染色体分离中发挥不同的作用。
In all eukaryotes, segregation of mitotic chromosomes requires their interaction with spindle microtubules. To dissect this interaction, we use live and fixed assays in the one-cell stage Caenorhabditis elegans embryo. We compare the consequences of depleting homologues of the centromeric histone CENP-A, the kinetochore structural component CENP-C, and the chromosomal passenger protein INCENP. Depletion of either CeCENP-A or CeCENP-C results in an identical “kinetochore null” phenotype, characterized by complete failure of mitotic chromosome segregation as well as failure to recruit other kinetochore components and to assemble a mechanically stable spindle. The similarity of their depletion phenotypes, combined with a requirement for CeCENP-A to localize CeCENP-C but not vice versa, suggest that a key step in kinetochore assembly is the recruitment of CENP-C by CENP-A–containing chromatin. Parallel analysis of CeINCENP-depleted embryos revealed mitotic chromosome segregation defects different from those observed in the absence of CeCENP-A/C. Defects are observed before and during anaphase, but the chromatin separates into two equivalently sized masses. Mechanically stable spindles assemble that show defects later in anaphase and telophase. Furthermore, kinetochore assembly and the recruitment of CeINCENP to chromosomes are independent. These results suggest distinct roles for the kinetochore and the chromosomal passengers in mitotic chromosome segregation.
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影响因子：
1.6
作者：
ALBERTSON, DG;THOMSON, JN
通讯作者：
THOMSON, JN
影响因子：
2.6
作者：
Albertson, Donna G.;Thomson, J. Nichol
通讯作者：
Thomson, J. Nichol
DOI：
10.1083/jcb.144.5.927
发表时间：
1999-03-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gönczy P;Schnabel H;Kaletta T;Amores AD;Hyman T;Schnabel R
通讯作者：
Schnabel R
影响因子：
11.6
作者：
Dawe, RK;Reed, LM;Hiatt, EN
通讯作者：
Hiatt, EN
影响因子：
7.8
作者：
Mackay, AM;Ainsztein, AM;Earnshaw, WC
通讯作者：
Earnshaw, WC