Dissection of cell division processes in the one cell stage Caenorhabditis elegans embryo by mutational analysis.
Dissection of cell division processes in the one cell stage Caenorhabditis elegans embryo by mutational analysis.
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DOI：
10.1083/jcb.144.5.927
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发表时间：
1999-03-08
期刊：
影响因子：
--
通讯作者：
Schnabel R
中科院分区：
文献类型：
--
作者：
Gönczy P;Schnabel H;Kaletta T;Amores AD;Hyman T;Schnabel R
为了确定复杂的真核生物细胞分裂过程所需的新组件，我们进行了广泛的突变分析，在一个细胞阶段秀丽隐杆线虫胚胎。该细胞的大尺寸和光学特性允许通过简单的微分干涉对比（DIC）显微镜观察活标本中的细胞分裂过程。我们已经筛选了大量的母源效应胚胎致死突变的染色体III与延时DIC视频显微镜。使用该测定，我们已经确定了34个基因座中的48个突变，这些突变是单细胞阶段胚胎中特定细胞分裂过程所需的。我们发现，突变属于不同的表型类，其中包括对应的原核迁移，旋转的中心体和相关的原核，纺锤体组装，染色体分离，后期纺锤体定位，和胞质分裂的过程。我们进一步分析了原核迁移突变体的间接免疫荧光显微镜使用抗体微管蛋白和ZYG-9，一个中心体标记。该分析表明，两个原核迁移位点是产生正常的微管阵列和四个中心体分离所需的。所有34个基因座都被定位在染色体III的不同区域，从而为它们的快速分子表征铺平了道路。我们的工作有助于建立一个细胞阶段C。线虫胚胎作为一个强大的后生动物模型系统解剖细胞分裂过程。
To identify novel components required for cell division processes in complex eukaryotes, we have undertaken an extensive mutational analysis in the one cell stage Caenorhabditis elegans embryo. The large size and optical properties of this cell permit observation of cell division processes with great detail in live specimens by simple differential interference contrast (DIC) microscopy. We have screened an extensive collection of maternal-effect embryonic lethal mutations on chromosome III with time-lapse DIC video microscopy. Using this assay, we have identified 48 mutations in 34 loci which are required for specific cell division processes in the one cell stage embryo. We show that mutations fall into distinct phenotypic classes which correspond, among others, to the processes of pronuclear migration, rotation of centrosomes and associated pronuclei, spindle assembly, chromosome segregation, anaphase spindle positioning, and cytokinesis. We have further analyzed pronuclear migration mutants by indirect immunofluorescence microscopy using antibodies against tubulin and ZYG-9, a centrosomal marker. This analysis revealed that two pronuclear migration loci are required for generating normal microtubule arrays and four for centrosome separation. All 34 loci have been mapped by deficiencies to distinct regions of chromosome III, thus paving the way for their rapid molecular characterization. Our work contributes to establishing the one cell stage C. elegans embryo as a powerful metazoan model system for dissecting cell division processes.
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影响因子：
2.7
作者：
CASSADA, R;ISNENGHI, E;VONEHRENSTEIN, G
通讯作者：
VONEHRENSTEIN, G
影响因子：
64.8
作者：
Heald, R;Tournebize, R;Karsenti, E
通讯作者：
Karsenti, E
影响因子：
56.9
作者：
HARTWELL, LH;CULOTTI, J;REID, BJ
通讯作者：
REID, BJ
影响因子：
19
作者：
Gonczy, P;Hyman, AA
通讯作者：
Hyman, AA
DOI：
10.1083/jcb.138.5.1055
发表时间：
1997-09-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gaglio T;Dionne MA;Compton DA
通讯作者：
Compton DA