Peonidin-3-O-glucoside and cyanidin increase osteoblast differentiation and reduce RANKL-induced bone resorption in transgenic medaka.
Peonidin-3-O-glucoside and cyanidin increase osteoblast differentiation and reduce RANKL-induced bone resorption in transgenic medaka.
复制标题
DOI：
10.1002/ptr.7271
复制
发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Mahady GB
中科院分区：
文献类型：
--
作者：
Ren Z;Raut NA;Lawal TO;Patel SR;Lee SM;Mahady GB
实验和临床研究表明花青素对骨骼健康有积极影响;然而，花青素改变成骨细胞和破骨细胞分化和功能的机制尚未完全了解。这项工作表明，膳食花青素和白藜芦醇增加了培养的人hFOB 1.19成骨细胞的增殖。此外，用1.0 μg/ml花青素和白藜芦醇处理hFOB成骨细胞的血清饥饿降低了细胞凋亡、Bax/Bcl-2比率、p53和HDAC 1表达，但增加了SIRT 1/3和PGC 1 α mRNA表达，表明线粒体和表观遗传调节。在Sp 7/osterix：mCherry转基因青鳉中，芍药苷-3-O-葡萄糖苷和白藜芦醇增加成骨细胞分化并增加Sp 7/osterix的表达。矢车菊素、芍药苷-3-O-葡萄糖苷和白藜芦醇在1-4 μg/ml的剂量下也减少了col 10 α1：nlGFP/rankl：HSE：CFP青鳉中RANKL诱导的异位破骨细胞形成和骨吸收。结果表明，矢车菊素和芍药苷-3-O-葡萄糖苷对骨具有合成代谢作用，增加成骨细胞增殖和分化，线粒体生物合成，并通过改变成骨细胞表观基因组。矢车菊素和芍药苷-3-O-葡萄糖苷也减少RANKL诱导的骨吸收的转基因青鳉模型的骨吸收。因此，芍药苷-3-O-葡萄糖苷和矢车菊素似乎都增加骨形成和减少骨丢失，这表明它们可以作为骨质疏松症和骨软化症的潜在治疗方法进行进一步研究。
Experimental and clinical studies suggest a positive impact of anthocyanins on bone health; however, the mechanisms of anthocyanins altering the differentiation and function of osteoblasts and osteoclasts are not fully understood. This work demonstrates that dietary anthocyanins and resveratrol increased proliferation of cultured human hFOB 1.19 osteoblasts. In addition, treatment of serum starvation of hFOB osteoblasts with anthocyanins and resveratrol at 1.0 μg/ml reduced apoptosis, the Bax/Bcl-2 ratio, p53, and HDAC1 expression, but increased SIRT1/3 and PGC1α mRNA expression, suggesting mitochondrial and epigenetic regulation. In Sp7/osterix:mCherry transgenic medaka, peonidin-3-O-glucoside and resveratrol increased osteoblast differentiation and increased the expression of Sp7/osterix. Cyanidin, peonidin-3-O-glucoside, and resveratrol also reduced RANKL-induced ectopic osteoclast formation and bone resorption in col10α1:nlGFP/rankl:HSE: CFP medaka in doses of 1–4 μg/ml. The results indicate that both cyanidin and peonidin-3-O-glucoside have anabolic effects on bone, increasing osteoblast proliferation and differentiation, mitochondrial biogenesis, and by altering the osteoblast epigenome. Cyanidin and peonidin-3-O-glucoside also reduced RANKL-induced bone resorption in a transgenic medaka model of bone resorption. Thus, peonidin-3-O-glucoside and cyanidin appear to both increase bone formation and reduce bone loss, suggesting that they be further investigated as potential treatments for osteoporosis and osteomalacia.
登录
查看更多内容
影响因子：
4.6
作者：
Chen X;Sun K;Jiao S;Cai N;Zhao X;Zou H;Xie Y;Wang Z;Zhong M;Wei L
通讯作者：
Wei L
影响因子：
4.8
作者：
Cohen-Kfir, Einav;Artsi, Hanna;Dresner-Pollak, Rivka
通讯作者：
Dresner-Pollak, Rivka
影响因子：
2
作者：
Imangali, Nurgul;Quang Tien Phan;Winkler, Christoph
通讯作者：
Winkler, Christoph
影响因子：
6.1
作者：
Bo S;Gambino R;Ponzo V;Cioffi I;Goitre I;Evangelista A;Ciccone G;Cassader M;Procopio M
通讯作者：
Procopio M
影响因子：
5.9
作者：
Arjmandi BH;Johnson SA;Pourafshar S;Navaei N;George KS;Hooshmand S;Chai SC;Akhavan NS
通讯作者：
Akhavan NS