Recent advances in activating silent biosynthetic gene clusters in bacteria.
Recent advances in activating silent biosynthetic gene clusters in bacteria.
复制标题
DOI：
10.1016/j.mib.2018.05.001
复制
发表时间：
2018-10
影响因子：
5.4
通讯作者：
Seyedsayamdost MR
中科院分区：
文献类型：
--
作者：
Mao D;Okada BK;Wu Y;Xu F;Seyedsayamdost MR
The explosion of microbial genome sequences has shown that bacteria harbor an immense, largely untapped potential for the biosynthesis of diverse natural products, which have traditionally served as an important source of pharmaceutical compounds. Most of the biosynthetic genes that can be detected bioinformatically are not, or only weakly, expressed under standard laboratory growth conditions. Herein we review three recent approaches that have been developed for inducing these so-called silent biosynthetic gene cluster: insertion of constitutively active promoters using CRISPR-Cas9, high-throughput elicitor screening for identification of small molecule inducers, and reporter-guided mutant selection for creation of overproducing strains. Together with strategies implemented previously, these approaches promise to unleash the products of silent gene clusters in years to come.
登录
查看更多内容
鉴定丁烯乙烯酯调节系统控制了Albus J1074链霉菌中的继发代谢。
影响因子：
4.6
作者：
Ahmed Y;Rebets Y;Tokovenko B;Brötz E;Luzhetskyy A
通讯作者：
Luzhetskyy A
影响因子：
5
作者：
Ochi, Kozo;Hosaka, Takeshi
通讯作者：
Hosaka, Takeshi
影响因子：
3.4
作者：
Bachmann, Brian O.;Van Lanen, Steven G.;Baltz, Richard H.
通讯作者：
Baltz, Richard H.
影响因子：
7.7
作者：
Rigali, Sebastien;Titgemeyer, Fritz;van Wezel, Gilles P.
通讯作者：
van Wezel, Gilles P.
影响因子：
11.9
作者：
Nett M;Ikeda H;Moore BS
通讯作者：
Moore BS