TREE1-EIN3-mediated transcriptional repression inhibits shoot growth in response to ethylene.
TREE1-EIN3-mediated transcriptional repression inhibits shoot growth in response to ethylene.
复制标题
Tree1-Ein3介导的转录抑制作用抑制了对乙烯的芽生长。
DOI：
10.1073/pnas.2018735117
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发表时间：
2020-11-17
影响因子：
11.1
通讯作者：
Qiao H
中科院分区：
文献类型：
--
作者：
Wang L;Ko EE;Tran J;Qiao H
乙烯是一种重要的植物激素，对于许多生理和发育过程如顶钩形成、芽和根生长、根形成、花衰老、脱落和果实成熟是必需的。乙烯反应中的转录激活已经被很好地研究。然而，乙烯反应中的转录抑制仍不清楚。在这里，通过数据分析，分子生物学和遗传学相结合的方法，我们发现，转录抑制因子TREE1与EIN 3相互作用，调节转录抑制，导致抑制芽生长响应乙烯。我们的工作提供了深入了解如何转录激活因子可以调整到抑制激素信号下游基因表达。乙烯是一种重要的植物激素，它调节植物的生长，其中主转录激活因子EIN 3（Ethylene Insensitive 3）介导的转录激活起着至关重要的作用。然而，EIN 3介导的转录抑制乙烯反应是未知的。我们在这里报告，EIN 3依赖的乙烯反应1（TREE1）的转录抑制因子与EIN 3相互作用，调节转录抑制，导致抑制芽生长响应乙烯。组织特异性转录组分析表明，大多数基因下调乙烯在芽，和DNA结合基序被确定为这是重要的转录抑制。TREE1与DNA基序结合，以EIN 3依赖性方式抑制基因表达。遗传验证表明，TREE1靶向基因的抑制导致芽生长的抑制。总的来说，这项工作建立了一个机制，转录抑制TREE1与EIN 3相互作用，抑制芽生长通过转录抑制响应乙烯。
Ethylene is an important plant hormone that is essential for many physiological and developmental processes such as apical hook formation, shoot and root growth, root nodulation, flower senescence, abscission, and fruit ripening. Transcriptional activation in the ethylene response has been well studied. However, the transcriptional repression in the ethylene response remains unclear. Here, by combined approaches of data analysis, molecular biology, and genetics, we found that a transcriptional repressor TREE1 interacts with EIN3 to regulate transcriptional repression, leading to an inhibition of shoot growth in response to ethylene. Our work provides insight into how the transcriptional activator can be tuned to repress downstream gene expression in hormone signals. Ethylene is an important plant hormone that regulates plant growth, in which the master transcriptionactivator EIN3 (Ethylene Insensitive 3)-mediated transcriptional activation plays vital roles. However, the EIN3-mediated transcriptional repression in ethylene response is unknown. We report here that a Transcriptional Repressor of EIN3-dependent Ethylene-response 1 (TREE1) interacts with EIN3 to regulate transcriptional repression that leads to an inhibition of shoot growth in response to ethylene. Tissue-specific transcriptome analysis showed that most of the genes are down-regulated by ethylene in shoots, and a DNA binding motif was identified that is important for this transcriptional repression. TREE1 binds to the DNA motif to repress gene expression in an EIN3-dependent manner. Genetic validation demonstrated that repression of TREE1-targeted genes leads to an inhibition of shoot growth. Overall, this work establishes a mechanism by which transcriptional repressor TREE1 interacts with EIN3 to inhibit shoot growth via transcriptional repression in response to ethylene.
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DOI：
10.1073/pnas.1316278111
发表时间：
2014-02-11
影响因子：
11.1
作者：
Franco-Zorrilla, Jose M.;Lopez-Vidriero, Irene;Solano, Roberto
通讯作者：
Solano, Roberto
影响因子：
7.2
作者：
EYAL, Y;MELLER, Y;FLUHR, R
通讯作者：
FLUHR, R
影响因子：
7.4
作者：
Kagale, Sateesh;Links, Matthew G.;Rozwadowski, Kevin
通讯作者：
Rozwadowski, Kevin
影响因子：
7.4
作者：
Binder, BM;Mortimore, LA;Bleecker, AB
通讯作者：
Bleecker, AB
影响因子：
56.9
作者：
Alonso, JM;Hirayama, T;Ecker, JR
通讯作者：
Ecker, JR