Identification of a biomass unaffected pale green mutant gene in Chinese cabbage (Brassica rapa L. ssp. pekinensis).
Identification of a biomass unaffected pale green mutant gene in Chinese cabbage (Brassica rapa L. ssp. pekinensis).
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白菜（Brassica rapa L. ssp）一个生物量不受影响的淡绿色突变基因的鉴定。学报)。
DOI：
10.1038/s41598-022-11825-1
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发表时间：
2022-05-11
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
叶绿素 (Chl) 是植物绿化中光合作用装置和色素的重要组成部分。叶色是大白菜重要的农艺和商业性状。在这项研究中，我们鉴定了大白菜中通过甲磺酸乙酯（EMS）诱变产生的浅绿色突变体 pgm。与野生型（FT）相比，pgm 的叶绿素含量较低，叶绿素 a/b 比较高，叶绿体结构不完善，非光化学猝灭较低。但其净光合速率和生物量没有显着差异。遗传分析表明，pgm 的淡绿色表型是由隐性核基因控制的，称为 Brpgm。我们应用BSR-Seq、连锁分析和全基因组重测序来定位Brpgm，并预测目标基因是BraA10g007770.3C (BrCAO)，它编码叶绿素加氧酶(CAO)。 Brcao测序结果显示，其第一个内含子的最后一个核苷酸从G变为A，导致其第二个CDS中的第一个核苷酸缺失并终止蛋白质翻译。 pgm中BrCAO表达上调，pgm中CAO酶活性显着降低。这些结果为探索BrCAO的功能和在大白菜中创造淡绿色变异提供了一种方法。
Chlorophyll (Chl) is an essential component of the photosynthetic apparatus and pigments in plant greening. Leaf color is an important agronomic and commercial trait of Chinese cabbage. In this study, we identified a pale green mutant pgm created by ethyl methane sulfonate (EMS) mutagenesis in Chinese cabbage. Compared with wild-type (FT), pgm had a lower Chl content with a higher Chl a/b ratio, imperfect chloroplast structure, and lower non-photochemical quenching. However, its net photosynthetic rate and biomass showed no significant differences. Genetic analysis revealed that the pale green phenotype of pgm was controlled by a recessive nuclear gene, designated as Brpgm. We applied BSR-Seq, linkage analysis, and whole-genome resequencing to map Brpgm and predicted that the target gene was BraA10g007770.3C (BrCAO), which encodes chlorophyllide a oxygenase (CAO). Brcao sequencing results showed that the last nucleotide of its first intron changed from G to A, causing the deletion of the first nucleotide in its second CDS and termination of the protein translation. The expression of BrCAO in pgm was upregulated, and the enzyme activity of CAO in pgm was significantly decreased. These results provide an approach to explore the function of BrCAO and create a pale green variation in Chinese cabbage.
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