Dietary triacylglycerol modulates sodium-dependent D-glucose transport, fluidity and fatty acid composition of rat small intestinal brush-border membrane.
Dietary triacylglycerol modulates sodium-dependent D-glucose transport, fluidity and fatty acid composition of rat small intestinal brush-border membrane.
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膳食三酰甘油调节大鼠小肠刷状缘膜的钠依赖性 D-葡萄糖转运、流动性和脂肪酸组成。
DOI：
10.1016/0005-2736(89)90433-1
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Baum,C
中科院分区：
文献类型：
--
作者：
Brasitus,TA;Dudeja,PK;Bolt,MJ;Sitrin,MD;Baum,C
Rats were maintained on nutritionally complete diets enriched in unsaturated (menhaden fish oil) or saturated (butter fat) triacylglycerols. After 4 weeks, the animals were killed, proximal small intestinal brush-border membranes were prepared, and examined and compared with respect to their lipid composition, molecular species of phosphatidylcholine, lipid fluidity and sodium-dependentd-glucose transport. Membranes prepared from the two dietary groups were found to possess similar ratios of cholesterol/phospholipid (mol/mol), sphingomyelin/phosphatidylcholine (mol/mol), and protein/lipid (w/w). In contrast to these findings, however, striking differences were noted in the total fatty acid compositions of these membranes. Plasma membranes prepared from animals fed the fish oil diet possessed higher percentages of saturated fatty acids as well as (n− 3) unsaturated fatty acids and lower percentages of monounsaturated and (n− 6) unsaturated fatty acids than those prepared from animals fed the butter fat diet. Analysis of the molecular species of phosphatidylcholine by HPLC, moreover, revealed that membranes from rats fed fish oil had higher levels of 16:0–20:5, 16:0–22:6 and 18:0–20:5 and lower levels of 18:0–18:2 and 16:0–18:1 than their butter fat counterparts. As assessed by steady-state fluorescence polarization, differential polarized phase fluorometric and excimer/monomer fluorescence intensity techniques using various fluorophores, the lipid fluidity of membranes from rats fed fish oil was also found to be significantly lower compared to membranes from rats fed butter fat. Finally, comparison of the kinetic parameters of Na+-dependentd-glucose transport revealed that fish oil-membrane vesicles had a higher maximum velocity (Vmax) than butter fat membrane vesicles but a similarKmfor glucose.
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DOI：
10.1016/0005-2736(88)90146-0
发表时间：
1988-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
T. Brasitus;P. Dudeja;E. S. Foster
通讯作者：
T. Brasitus;P. Dudeja;E. S. Foster
酒精抑制脂肪细胞基础和胰岛素刺激的葡萄糖摄取并增加膜脂流动性。
DOI：
--
发表时间：
1982
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
R. Sauerheber;J. Esgate;Chris E Kuhn
通讯作者：
Chris E Kuhn
Na 偶联 D-葡萄糖摄取、Mg2-ATP 酶和蔗糖酶对正脂肪醇诱导的刷状缘膜囊泡流动性扰动的敏感性。
DOI：
--
发表时间：
1984
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
Y. Fernandez;R. Boigegrain;C. Cambon;S. Mitjavila
通讯作者：
S. Mitjavila
影响因子：
2.9
作者：
C. Stubbs;W. M. Tsang;J. Belin;A. D. Smith;S. M. Johnson
通讯作者：
S. M. Johnson
DOI：
--
发表时间：
1953
期刊：
Journal of Laboratory and Clinical Medicine
影响因子：
--
作者：
A. Zlatkis;A. Zlatkis;B. Zak;B. Zak;A. Boyle;A. Boyle
通讯作者：
A. Boyle