Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae.
Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae.
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DOI：
10.1016/j.celrep.2022.110494
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发表时间：
2022-03-08
期刊：
影响因子：
8.8
通讯作者：
Potter CJ
中科院分区：
文献类型：
--
作者：
Maguire SE;Afify A;Goff LA;Potter CJ
蚊子根据嗅觉受体神经元（orn）上表达的气味受体（ORs）的活性定位和接近人类。冈比亚按蚊嗅觉发生实验表明，AgOR （AgOR2）异位表达可抑制表达神经元的活性。这与黑腹果蝇的研究形成了对比，在黑腹果蝇的研究中，非天然嗅觉嗅觉的异位表达在不干扰天然嗅觉生理的情况下赋予异位神经元反应。RNA-seq分析将野生型天线与异位表达AgOR2的天线进行比较，结果表明，几乎所有AgOR转录本都显著下调（AgOR2除外）。另外的实验表明，AgOR2蛋白而不是mRNA介导了这种下调。通过原位杂交，我们发现AgOR基因选择在成年期是活跃的，而AgOR2的表达抑制了AgORs在这一后期的开启。我们的研究表明，与果蝇相比，按蚊的orn对目前尚未探索的AgOR调节机制敏感。Maguire等人发现一种嗅觉受体的异位表达可以下调蚊子嗅觉神经元中内源性气味受体的转录。蚊子嗅觉神经元的气味受体表达持续到成虫阶段，并且对外源性嗅觉受体表达特别敏感。
Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed on olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that the ectopic expression of an AgOR (AgOR2) in ORNs dampened the activity of the expressing neuron. This contrasts with studies in Drosophila melanogaster in which the ectopic expression of non-native ORs in ORNs confers ectopic neuronal responses without interfering with native olfactory physiology. RNA-seq analyses comparing wild-type antennae to those ectopically expressing AgOR2 in ORNs indicated that nearly all AgOR transcripts were significantly downregulated (except for AgOR2). Additional experiments suggest that AgOR2 protein rather than mRNA mediates this downregulation. Using in situ hybridization, we find that AgOR gene choice is active into adulthood and that AgOR2 expression inhibits AgORs from turning on at this late stage. Our study shows that the ORNs of Anopheles mosquitoes (in contrast to Drosophila) are sensitive to a currently unexplored mechanism of AgOR regulation. Maguire et al. discover that the ectopic expression of an olfactory receptor can downregulate the transcription of endogenous odorant receptors in mosquito olfactory neurons. The onset of mosquito odorant-receptor expression by an olfactory neuron continues into adult stages, and is particularly sensitive to exogenous olfactory reception expression.
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