Synthesis and Characterization of Carboxymethylcellulose-Methacrylate Hydrogel Cell Scaffolds.
Synthesis and Characterization of Carboxymethylcellulose-Methacrylate Hydrogel Cell Scaffolds.
复制标题
DOI：
10.3390/polym2030252
复制
发表时间：
2010-09-01
期刊：
影响因子：
5
通讯作者：
Leach JB
中科院分区：
文献类型：
--
作者：
Reeves R;Ribeiro A;Lombardo L;Boyer R;Leach JB
许多碳水化合物由于其亲水性、可降解性和可用于修饰的化学基团的可用性而对组织工程应用构成优势。例如，羧甲基纤维素（CMC）是可被纤维素酶降解的水溶性纤维素衍生物。虽然这种酶不是由哺乳动物细胞合成的，但纤维素酶和来自CMC降解的片段是生物相容的。考虑到这一点，我们创造了生物相容的，选择性可降解的CMC基水凝胶，在常规培养中是稳定的，但当暴露于外源性纤维素酶时会降解。CMC-甲基丙烯酸酯和聚乙二醇二甲基丙烯酸酯（PEG-DM）的溶液共交联形成稳定的水凝胶，我们发现，更大的CMC-甲基丙烯酸酯含量导致增加的凝胶溶胀，蛋白质扩散和降解率由纤维素酶，以及降低凝胶剪切模量。用粘附肽RGD修饰的CMC-甲基丙烯酸酯/PEG-DM凝胶支持成纤维细胞粘附和活力。我们的结论是，基于CMC-甲基丙烯酸酯的水凝胶适合于生物工程应用，其中选择性降解性可能是有利的，如细胞支架或控释装置。
Many carbohydrates pose advantages for tissue engineering applications due to their hydrophilicity, degradability, and availability of chemical groups for modification. For example, carboxymethylcellulose (CMC) is a water-soluble cellulose derivative that is degradable by cellulase. Though this enzyme is not synthesized by mammalian cells, cellulase and the fragments derived from CMC degradation are biocompatible. With this in mind, we created biocompatible, selectively degradable CMC-based hydrogels that are stable in routine culture, but degrade when exposed to exogenous cellulase. Solutions of CMC-methacrylate and polyethylene glycol dimethacrylate (PEG-DM) were co-crosslinked to form stable hydrogels; we found that greater CMC-methacrylate content resulted in increased gel swelling, protein diffusion and rates of degradation by cellulase, as well as decreased gel shear modulus. CMC-methacrylate/PEG-DM gels modified with the adhesive peptide RGD supported fibroblast adhesion and viability. We conclude that hydrogels based on CMC-methacrylate are suitable for bioengineering applications where selective degradability may be favorable, such as cell scaffolds or controlled release devices.
登录
查看更多内容
影响因子：
2.6
作者：
Siddiqui, KS;Azhar, MJ;Rajoka, MJ
通讯作者：
Rajoka, MJ
DOI：
10.1073/pnas.74.9.3874
发表时间：
1977-01-01
影响因子：
11.1
作者：
CARRINO, D;GERSHMAN, H
通讯作者：
GERSHMAN, H
影响因子：
3
作者：
Lee, JH;Nho, YC;Son, TI
通讯作者：
Son, TI
影响因子：
10.8
作者：
Rodríguez, R;Alvarez-Lorenzo, C;Concheiro, A
通讯作者：
Concheiro, A
DOI：
10.1002/jbm.820230110
发表时间：
1989-01-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
MIYAMOTO, T;TAKAHASHI, S;NOISHIKI, Y
通讯作者：
NOISHIKI, Y