Evaluation of Merkel Cell Polyomavirus DNA in Tissue Samples from Italian Patients with Diagnosis of MCC.
Evaluation of Merkel Cell Polyomavirus DNA in Tissue Samples from Italian Patients with Diagnosis of MCC.
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DOI：
10.3390/v13010061
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发表时间：
2021-01-05
期刊：
影响因子：
--
通讯作者：
Pietropaolo V
中科院分区：
文献类型：
--
作者：
Prezioso C;Carletti R;Obregon F;Piacentini F;Manicone AM;Soda G;Moens U;Di Gioia C;Pietropaolo V
由于默克尔细胞癌（MCC）的发病率在过去10年中显著增加，并且人们认识到默克尔细胞多瘤病毒（MCPyV）和紫外线（UV）辐射代表两种不同的病因学输入，尽管具有不同的预后，但具有相似的临床、组织病理学和预后特征，因此本研究调查了皮肤和淋巴结中MCPyV的检测以及MCC的组织学诊断。从存档标本中取出福尔马林固定石蜡包埋组织（FFPE），扩增MCPyV非编码控制区（NCCR）和病毒衣壳蛋白1（VP1）序列并测序。结果提供了一个有趣的观察有关的差异之间的MCPyV DNA的状态在原发性和转移性位点：事实上，在所有的情况下，其中原发性和转移性病变进行了研究，MCPyV DNA仅在原发性病变检测。我们的数据进一步支持了其他作者提出的“打了就跑”理论，并可能导致推测，在一些MCC中，病毒仅是肿瘤起始过程所必需的，进一步的突变可能使肿瘤独立于病毒。与MCPyV MCC350分离株相比，在NCCR中检测到很少的点突变，在VP1序列中仅观察到沉默突变。为了明确确定MCPyV在恶性肿瘤中的作用，需要进行额外的良好对照研究，并应检查更大的队列。
Because the incidence of Merkel cell carcinoma (MCC) has increased significantly during the last 10 years and it is recognized that Merkel cell polyomavirus (MCPyV) and ultraviolet (UV) radiation represent two different etiological inputs sharing clinical, histopathological, and prognostic similar features, although with different prognosis, this study investigated the detection of MCPyV in skin and lymph nodes with histological diagnosis of MCC. Formalin-fixed paraffin-embedded tissue (FFPE) were retrieved from archived specimens and MCPyV non-coding control region (NCCR) and viral capsid protein 1 (VP1) sequences were amplified and sequenced. Results provide an interesting observation concerning the discrepancy between the MCPyV DNA status in primary and metastatic sites: in fact, in all cases in which primary and metastatic lesions were investigated, MCPyV DNA was detected only in the primary lesions. Our data further support the “hit-and-run” theory, also proposed by other authors, and may lead to speculation that in some MCCs the virus is only necessary for the process of tumor initiation and that further mutations may render the tumor independent from the virus. Few point mutations were detected in the NCCR and only silent mutations were observed in the VP1 sequence compared to the MCPyV MCC350 isolate. To unequivocally establish a role of MCPyV in malignancies, additional well-controlled investigations are required, and larger cohorts should be examined.
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影响因子：
11.2
作者：
Harms PW;Vats P;Verhaegen ME;Robinson DR;Wu YM;Dhanasekaran SM;Palanisamy N;Siddiqui J;Cao X;Su F;Wang R;Xiao H;Kunju LP;Mehra R;Tomlins SA;Fullen DR;Bichakjian CK;Johnson TM;Dlugosz AA;Chinnaiyan AM
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Chinnaiyan AM
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11.3
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You J
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3.7
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通讯作者：
Erovic, Boban M.
影响因子：
10.3
作者：
Sihto, Harri;Kukko, Heli;Joensuu, Heikki
通讯作者：
Joensuu, Heikki
影响因子：
11.5
作者：
Knepper, Todd C.;Montesion, Meagan;Brohl, Andrew S.
通讯作者：
Brohl, Andrew S.