The specificity protein 3 (SP3) gene in ducks (Anas platyrhynchos): cloning, characterization and expression during viral infection
The specificity protein 3 (SP3) gene in ducks (Anas platyrhynchos): cloning, characterization and expression during viral infection
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鸭（Anas platyrhynchos）特异性蛋白 3 (SP3) 基因：病毒感染期间的克隆、表征和表达
DOI：
10.1080/10495398.2020.1740240
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发表时间：
2020-03
影响因子：
3.7
通讯作者：
Chen Guohong
中科院分区：
文献类型：
--
作者：
Cao Zhengfeng;Gao Wen;Gu Tiantian;Huo Weiran;Zhang Yang;Zhang Yu;Xu Qi;Chen Guohong
Abstract Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, including a 111 base pair (bp) 5′-untranslated region (UTR), 215 bp 3′-UTR, and 2142 bp open reading frame (ORF), which encoded a 713 amino acid (AA) strongly conserved with Avian SP3. Tissue specificity analysis demonstrated that duSP3 was constitutively expressed in the eight tissues tested: liver, spleen, lung, heart, kidney, thymus, breast, and leg; and low expression levels were observed in all tissues, except the spleen and thymus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that duSP3 expression rapidly increased in vitro after stimulation with both the hepatitis virus (DHV-1) and polyriboinosinic polyribocytidylic acid (poly(I:C)). However, the expression under these treatments varied in kidney and liver tissues; in the liver, duSP3 increased significantly at 36 h after the DHV-1 treatment and peaked at 72 h after poly(I:C) stimulation. These results suggested that SP3 may play a positive role in immune responses against viral infections in ducks.
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影响因子：
29.4
作者：
Kim CK;He P;Bialkowska AB;Yang VW
通讯作者：
Yang VW
影响因子：
3.7
作者：
Xu Q;Chen Y;Zhao WM;Huang ZY;Zhang Y;Li X;Tong YY;Chang GB;Duan XJ;Chen GH
通讯作者：
Chen GH
影响因子：
5.2
作者：
Li N;Lv C;Yue R;Shi Y;Wei L;Chai T;Liu S
通讯作者：
Liu S
DOI：
--
发表时间：
2006
期刊：
Chinese Journal of Biochemistry and Molecular Biology
影响因子：
--
作者：
Xue Li
通讯作者：
Xue Li
影响因子：
32.4
作者：
Potocnik, AJ;Brakebusch, C;Fässler, R
通讯作者：
Fässler, R