Carbon nanotubes allow capture of krypton, barium and lead for multichannel biological X-ray fluorescence imaging.
Carbon nanotubes allow capture of krypton, barium and lead for multichannel biological X-ray fluorescence imaging.
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DOI：
10.1038/ncomms13118
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发表时间：
2016-10-26
影响因子：
16.6
通讯作者：
Davis, Benjamin G.
中科院分区：
文献类型：
--
作者：
Serpell, Christopher J.;Rutte, Reida N.;Geraki, Kalotina;Pach, Elzbieta;Martincic, Markus;Kierkowicz, Magdalena;De Munari, Sonia;Wals, Kim;Raj, Ritu;Ballesteros, Belen;Tobias, Gerard;Anthony, Daniel C.;Davis, Benjamin G.
许多成像技术帮助了人们对原位生物学研究的渴望。其中，x射线荧光（XRF）映射允许以多通道方式观察元素分布。然而，XRF成像没有得到充分利用，部分原因是如果没有潜在的细胞“蓝图”，就难以解释地图；这可以使用造影剂。碳纳米管（CNTs）可以填充各种无机材料，因此如果将生物上不存在的元素包裹起来，就可以用作“造影剂”。在这里，我们证明了可以制备密封的单壁碳纳米管，填充铅、钡甚至氪，并在外部用肽修饰以提供对亚细胞靶点的亲和力。该试剂能够在多路XRF绘图中突出显示特定的细胞器，并且原则上是用于此和其他生物成像模式的通用和通用工具。x射线荧光（XRF）成像的生物学应用目前仅限于绘制组织中天然存在的元素。在这里，作者将有毒元素封装在功能化的单壁纳米管中，并将其用作无毒的XRF造影剂，用于成像特定的细胞器。
The desire to study biology in situ has been aided by many imaging techniques. Among these, X-ray fluorescence (XRF) mapping permits observation of elemental distributions in a multichannel manner. However, XRF imaging is underused, in part, because of the difficulty in interpreting maps without an underlying cellular ‘blueprint'; this could be supplied using contrast agents. Carbon nanotubes (CNTs) can be filled with a wide range of inorganic materials, and thus can be used as ‘contrast agents' if biologically absent elements are encapsulated. Here we show that sealed single-walled CNTs filled with lead, barium and even krypton can be produced, and externally decorated with peptides to provide affinity for sub-cellular targets. The agents are able to highlight specific organelles in multiplexed XRF mapping, and are, in principle, a general and versatile tool for this, and other modes of biological imaging. Biological applications of x-ray fluorescence (XRF) imaging are currently limited to mapping naturally occurring elements in tissues. Here, the authors encapsulate toxic elements in functionalized single walled nanotubes, and use them as non-toxic XRF contrast agents for imaging specific cellular organelles.
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