Seroprevalence of Neospora caninum-specific antibodies in German breeding bitches.
Seroprevalence of Neospora caninum-specific antibodies in German breeding bitches.
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DOI：
10.1186/s13071-018-2683-1
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发表时间：
2018-02-17
影响因子：
3.2
通讯作者：
Wehrend A
中科院分区：
文献类型：
--
作者：
Villagra-Blanco R;Angelova L;Conze T;Schares G;Bärwald A;Taubert A;Hermosilla C;Wehrend A
犬新孢子虫是一种细胞内专性顶复体寄生虫，可引起犬的多系统病变。作为最终宿主和宿主，狗排泄对环境有抗性的卵囊。繁殖母犬代表易感犬群，受感染母犬可能通过经胎盘传播这种寄生虫。共采集218份德国种母犬血清样本，以确定是否存在N。犬的使用商业间接酶联免疫吸附试验（ELISA）检测抗体16（7.33%）的母狗。免疫印迹分析证实了ELISA检测的所有血清阳性样品，证明动物感染了N。犬的对主人进行了关于品种、年龄、环境、类型、疫苗状态、喂养习惯和生殖障碍的采访。血清反应呈阳性的动物年龄在2至7岁之间;其中3只被关在狗舍中，其他是家养狗，其中一只还是猎犬。四只血清反应阳性母犬的主人报告了一次妊娠，而其他十二只母犬记录了多次妊娠。14只母犬定期接种疫苗，6只母犬喂新鲜生肉。虽然结果证实了N.犬血清阳性的德国繁殖母犬，需要进一步的流行病学和监测研究，以补充我们关于德国这一特定犬种群中新孢子虫病现状的发现。
Neospora caninum is an intracellular obligate apicomplexan parasite responsible for multisystemic lesions in dogs. Being definitive hosts and reservoirs, dogs excrete environmentally resistant oocysts. Breeding bitches represent a susceptible dog group and infected bitches may spread this parasite through transplacental transmission. A total of 218 serum samples of German breeding bitches were collected to determine the presence of N. caninum. Antibodies were detected in 16 (7.33%) bitches using a commercial indirect enzyme-linked immunosorbent assay (ELISA). Immunoblotting analysis confirmed all seropositive samples detected by ELISA, proving that the animals were infected with N. caninum. The owners were interviewed regarding breed, age, environment, type, vaccine status, feeding habits and the presence of reproductive disorders. Seropositive animals were between the ages of two to seven years; three of them were kept in kennels while the others were household dogs, one of which was additionally a hunting dog. Owners of four seropositive bitches reported one gestation, while multiple pregnancies had been recorded for the other twelve bitches. Fourteen bitches were regularly vaccinated and six were fed with fresh raw meat. Although the results confirmed a low incidence of N. caninum seropositive German breeding bitches, further epidemiological and surveillance studies are required to complement our findings regarding the current situation of neosporosis in this specific canine population of Germany.
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影响因子：
1.3
作者：
Gondim, LFP;McAllister, MM;Nelson, ME
通讯作者：
Nelson, ME
影响因子：
2.6
作者：
Gavrea, R.;Mircean, V.;Cozma, V.
通讯作者：
Cozma, V.
影响因子：
2.2
作者：
Barber, JS;Trees, AJ
通讯作者：
Trees, AJ
影响因子：
2.6
作者：
Collantes-Fernandez, E.;Gomez-Bautista, A.;Ortega-Mora, L. M.
通讯作者：
Ortega-Mora, L. M.
影响因子：
2.4
作者：
Ferroglio, E.;Pasino, M.;Trisciuoglio, A.
通讯作者：
Trisciuoglio, A.