Open software platform for automated analysis of paper-based microfluidic devices

Open software platform for automated analysis of paper-based microfluidic devices
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用于纸基微流体装置自动分析的开放软件平台

DOI:
10.1038/s41598-020-67639-6
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发表时间:
2020
期刊:
影响因子:
4.6
通讯作者:
Mace, Charles R.
Mace, Charles R.
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Parker, Rayleigh W.;Wilson, Daniel J.;Mace, Charles R.

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开发进行比色测量的纸基微流体装置需要定量图像分析。由于纸基微流体装置的设计几何形状未标准化,因此对信号强度规则间隔区域(例如孔板的方法)进行批量测量的传统方法不能用于量化大多数这些装置的信号。为了简化设备开发过程,我们开发了一个名为 ColorScan 的开源程序,它可以自动识别和测量设备图像中包含信号的区域,无论输出区域的几何形状或空间排列如何。该程序以与标准手动方法相同的精度测量颜色强度,可以快速处理扫描的设备图像,同时测量识别的输出区域,并有效管理测量结果,从而消除耗时且依赖于用户的图像处理程序的要求。
Development of paper-based microfluidic devices that perform colorimetric measurements requires quantitative image analysis. Because the design geometries of paper-based microfluidic devices are not standardized, conventional methods for performing batch measurements of regularly spaced areas of signal intensity, such as those for well plates, cannot be used to quantify signal from most of these devices. To streamline the device development process, we have developed an open-source program called ColorScan that can automatically recognize and measure signal-containing zones from images of devices, regardless of output zone geometry or spatial arrangement. This program, which measures color intensity with the same accuracy as standard manual approaches, can rapidly process scanned device images, simultaneously measure identified output zones, and effectively manage measurement results to eliminate requirements for time-consuming and user-dependent image processing procedures.
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