Human CST has independent functions during telomere duplex replication and C-strand fill-in.
Human CST has independent functions during telomere duplex replication and C-strand fill-in.
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DOI：
10.1016/j.celrep.2012.10.007
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发表时间：
2012-11-29
期刊：
影响因子：
8.8
通讯作者：
Price CM
中科院分区：
文献类型：
--
作者：
Wang F;Stewart JA;Kasbek C;Zhao Y;Wright WE;Price CM
Human CST (CTC1-STN1-TEN1) is an RPA-like complex that is needed for efficient replication through the telomere duplex and genome-wide replication restart after fork stalling. Here we show that STN1/CST has a second function in telomere replication during G-overhang maturation. Analysis of overhang structure after STN1 depletion revealed normal kinetics for telomerase-mediated extension in S-phase but a delay in subsequent overhang shortening. This delay resulted from a defect in C-strand fill-in. Short telomeres exhibited the fill-in defect but normal telomere duplex replication, indicating that STN1/CST functions independently in these processes. Our work also indicates that the requirement for STN1/CST in telomere duplex replication correlates with increasing telomere length and replication stress. Our results provide the first direct evidence that STN1/CST participates in C-strand fill-in. They also demonstrate that STN1/CST participates in two mechanistically separate steps during telomere replication and identify CST as a novel replication factor that solves diverse replication-associated problems.
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影响因子：
64.5
作者：
Zhao Y;Sfeir AJ;Zou Y;Buseman CM;Chow TT;Shay JW;Wright WE
通讯作者：
Wright WE
影响因子：
64.5
作者：
Wu P;Takai H;de Lange T
通讯作者：
de Lange T
DOI：
10.1074/jbc.m111.263723
发表时间：
2012-01-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nakaoka H;Nishiyama A;Saito M;Ishikawa F
通讯作者：
Ishikawa F
影响因子：
64.5
作者：
Sfeir A;Kosiyatrakul ST;Hockemeyer D;MacRae SL;Karlseder J;Schildkraut CL;de Lange T
通讯作者：
de Lange T
DOI：
10.1016/j.mrfmmm.2011.08.011
发表时间：
2012-02-01
影响因子：
2.3
作者：
Stewart, Jason A.;Chaiken, Mary F.;Wang, Feng;Price, Carolyn M.
通讯作者：
Price, Carolyn M.