First molecular evidence of Hepatozoon canis infection in red foxes and golden jackals from Hungary.
First molecular evidence of Hepatozoon canis infection in red foxes and golden jackals from Hungary.
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DOI：
10.1186/1756-3305-7-303
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发表时间：
2014-07-02
影响因子：
3.2
通讯作者：
Baneth G
中科院分区：
文献类型：
--
作者：
Farkas R;Solymosi N;Takács N;Hornyák Á;Hornok S;Nachum-Biala Y;Baneth G
最近，在匈牙利南部的牧羊犬、猎狗和流浪狗中发现了犬肝虫感染，该地区被认为没有广义血红扇头蜱，靠近克罗地亚边境。本研究的目的是获得关于赤狐和/或金豺可能在H.匈牙利的犬。使用常规PCR从16个县231个地点射杀的334只狐狸和靠近克罗地亚的西南部两个县9个地点射杀的15只金豺狼的血液样本中扩增了肝虫18 S rRNA基因的666 bp长片段。在第一次PCR阳性的一些样品中进行第二次PCR检测，以扩增肝虫属的18 S rRNA基因的较大片段（约1500 bp）。做进一步的系统发育分析在9个县30个地点的犬中检测到肝体虫感染。PCR阳性率为8.0%，95%CI：5-11%;阳性率为60%，95%CI：33-81%。从12只狐狸和7只豺中获得了犬肝吸虫的基因序列。16只动物的DNA序列与H.其中两个序列与意大利狐狸有99%的相似性。在西南两县，半数（13/26）的赤狐和所有的金豺被射杀。这是首次报道H.在匈牙利的赤狐和金黄色胡狼体内发现了犬血蜱，认为该动物体内没有H.卡尼斯河多血的虽然没有R。在感染或未感染的野生犬科动物身上发现了宽血吸虫，匈牙利发现的犬肝虫病可能暗示了宽血吸虫的范围。广义上的血色已经降临这个国家。
Recently, Hepatozoon canis infection has been detected among shepherd, hunting and stray dogs in the southern part of Hungary, which is considered to be free of Rhipicephalus sanguineus sensu lato and close to the border with Croatia. The aim of this study was to acquire information on the possibility that red foxes and/or golden jackals could play a role in the appearance and spread of H. canis in Hungary. A conventional PCR was used to amplify a 666 bp long fragment of the Hepatozoon 18S rRNA gene from blood samples collected from 334 foxes shot in 231 locations in 16 counties and 15 golden jackals shot in 9 locations in two southwestern counties close to Croatia. A second PCR assay was performed in some of the samples positive by the first PCR to amplify a larger segment (approximately 1500 bp) of the 18S rRNA gene of Hepatozoon spp. for further phylogenetic analysis. Hepatozoon infection was detected in canids shot in 30 locations and 9 counties. Altogether 26 foxes (8.0%, 95% CI: 5-11%) and 9 jackals (60%, 95% CI: 33-81%) were PCR positive. Hepatozoon canis sequences were obtained from 12 foxes and 7 jackals. DNA sequences from 16 animals were 99-100% similar to H. canis from Croatian foxes or dogs while two of the sequences were 99% similar to an Italian fox. Half (13/26) of the infected red foxes and all golden jackals were shot in the two southwestern counties. This is the first report on molecular evidence of H. canis in red foxes (Vulpes vulpes) and golden jackals (Canis aureus) from Hungary, which is considered free from the tick vector of H. canis, R. sanguineus. Although no R. sanguineus sensu lato had been found on infected or non-infected wild canids, the detection of authochnous canine hepatozoonosis in Hungary might imply that the range of R. sanguineus sensu lato has reached this country.
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影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
2.6
作者：
de Miranda, Renata Lima;de Castro, Jacqueline Ribeiro;Baneth, Gad
通讯作者：
Baneth, Gad
影响因子：
3.2
作者：
Duscher, Georg Gerhard;Kuebber-Heiss, Anna;Suchentrunk, Franz
通讯作者：
Suchentrunk, Franz
影响因子：
1.3
作者：
Criado-Fornelio, A;Ruas, JL;Barba-Carretero, JC
通讯作者：
Barba-Carretero, JC
影响因子：
3.2
作者：
Cardoso, Luis;Cortes, Helder C. E.;Baneth, Gad
通讯作者：
Baneth, Gad