Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.
Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.
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DOI：
10.1016/j.bioactmat.2023.09.017
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发表时间：
2024-02
影响因子：
18.9
通讯作者：
中科院分区：
文献类型：
--
作者：
组织工程和再生医学已经通过使用工程生物材料和支架在组织和器官的修复和再生方面显示出潜力。然而，目前的结构在复制复杂的原生微环境和实现最佳再生能力和功能恢复方面面临局限性。为了解决这些挑战，利用脱细胞组织和细胞源性细胞外基质（ECM）已经成为一种很有前途的方法。这些具有生物相容性和生物活性的生物材料可以被设计成多孔支架和移植物，在体外和体内模拟天然组织或器官微环境的结构和组成方面。生物活性dECM材料提供了一种独特的组织特异性微环境，可以调节和引导细胞过程，从而增强再生治疗。本文综述了脱细胞组织源性和细胞源性生物材料和生物墨水在组织工程和再生医学领域的最新进展。我们讨论了进一步改进脱细胞方法和技术的必要性，以模仿天然组织和器官的方式保留dECM产品的结构，生物和物理化学特征。本文强调了dECM生物材料通过趋化效应刺激原位组织修复的潜力，以促进生长因子和无细胞组织工程策略的发展。文章还指出了开发适用于脱细胞生物材料和移植物及其转化为临床产品的灭菌和保存方法的挑战和机遇。具有组织特异性微环境、结构和力学的脱细胞组织/细胞来源的非免疫生物材料脱细胞移植物通过趋化效应和天然GFs的存在刺激原位组织再生的潜力。使用dECM生物材料作为“组织墨水”用于工程移植物的生物制造。灭菌/保存的重要性和建立临床翻译的良好生产规范标准的必要性强调了最近的发展，当代的挑战，以及再生治疗中基于脱细胞的移植物。
Tissue engineering and regenerative medicine have shown potential in the repair and regeneration of tissues and organs via the use of engineered biomaterials and scaffolds. However, current constructs face limitations in replicating the intricate native microenvironment and achieving optimal regenerative capacity and functional recovery. To address these challenges, the utilization of decellularized tissues and cell-derived extracellular matrix (ECM) has emerged as a promising approach. These biocompatible and bioactive biomaterials can be engineered into porous scaffolds and grafts that mimic the structural and compositional aspects of the native tissue or organ microenvironment, both in vitro and in vivo. Bioactive dECM materials provide a unique tissue-specific microenvironment that can regulate and guide cellular processes, thereby enhancing regenerative therapies. In this review, we explore the emerging frontiers of decellularized tissue-derived and cell-derived biomaterials and bio-inks in the field of tissue engineering and regenerative medicine. We discuss the need for further improvements in decellularization methods and techniques to retain structural, biological, and physicochemical characteristics of the dECM products in a way to mimic native tissues and organs. This article underscores the potential of dECM biomaterials to stimulate in situ tissue repair through chemotactic effects for the development of growth factor and cell-free tissue engineering strategies. The article also identifies the challenges and opportunities in developing sterilization and preservation methods applicable for decellularized biomaterials and grafts and their translation into clinical products. Decellularized tissue-/cell-derived non-immune biomaterials with tissue-specific microenvironment, structure and mechanics Decellularized grafts potential to stimulate in situ tissue regeneration through chemotactic effects and presence of native GFs. The use of dECM biomaterials as “Tissue-Inks” for the bio-fabrication of engineered grafts. Sterilization/preservation importance and need to establish Good Manufacturing Practice standards for clinical translation Highlights recent developments, contemporary challenges, and decellularized-based grafts for regenerative therapies.
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