Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
Functional characterization of detergent-decellularized equine tendon extracellular matrix for tissue engineering applications.
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DOI：
10.1371/journal.pone.0064151
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Youngstrom DW;Barrett JG;Jose RR;Kaplan DL
天然细胞外基质由于其固有的功能特性，为工程化替代骨科组织提供了许多独特的优势。本研究的目的是优化生物衍生的肌腱组织工程支架使用马屈肌腱浅。我们研究了支架组成和超微结构的变化，响应于几个机械，去污剂和酶脱细胞方案，使用显微镜技术和一组生化测定，以评估总蛋白，胶原蛋白，糖胺聚糖和脱氧核糖核酸含量。还用静态间充质干细胞（MSC）培养评估了生物相容性。组合实施冻融循环、在2%十二烷基硫酸钠（SDS）中孵育、胰蛋白酶消化、用DNase-I处理和乙醇灭菌产生了无细胞毒性的生物材料，该生物材料不含明显的残留细胞碎片，生物力学特性无显著改变。这些脱细胞肌腱支架（脱细胞肌腱支架）适用于复杂的组织工程应用，因为它们为细胞培养提供了一个干净的石板，同时保持了天然的三维结构。
Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.
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DOI：
10.3109/02844318909075117
发表时间：
1989-01-01
影响因子：
--
作者：
ABRAHAMSSON, SO;LUNDBORG, G;LOHMANDER, LS
通讯作者：
LOHMANDER, LS
影响因子：
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作者：
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通讯作者：
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影响因子：
82.9
作者：
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通讯作者：
Young, Marian F.
影响因子：
4
作者：
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通讯作者：
Ginis, Irene
影响因子：
2.4
作者：
Cebotari, Serghei;Tudorache, Igor;Lichtenberg, Artur
通讯作者：
Lichtenberg, Artur