OsMADS25 regulates root system development via auxin signalling in rice
OsMADS25 regulates root system development via auxin signalling in rice
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OsMADS25 通过生长素信号调节水稻根系发育
DOI：
10.1111/tpj.14007
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发表时间：
2018-07
期刊：
影响因子：
7.2
通讯作者：
Huang Junli
中科院分区：
文献类型：
--
作者：
Zhang Guopeng;Xu Ning;Chen Hongli;Wang Guixue;Huang Junli
植物激素生长素是植物根系发育所必需的。OsMADS25是水稻AGL17分支MADS-box基因的同源物。尽管最近取得了一些进展，但OsMADS25调控根发育的分子机制还不是很清楚。目前还不清楚OsMADS25是否通过生长素信号来调节根的发育。在本研究中，我们研究了OsMADS25在根发育中的作用，并研究了OsMADS25调控水稻根系发育的信号通路。OsMADS25过表达显著增加，但RNAi基因沉默抑制了主根(PR)长度和侧根(LR)密度。此外，OsMADS25还促进了LR的发育，以响应NO3-。进一步的研究表明，OsMADS25通过促进生长素的生物合成和运输，增加了生长素在根系中的积累，同时也减少了生长素的降解，从而刺激了根的发育。更重要的是，OsMADS25被发现通过与OsIAA14启动子区域的Carg-box结合直接调节OsIAA14的表达，Carg-box编码生长素信号的AUX/吲哚乙酸(IAA)转录抑制物。生长素水平的升高和OsIAA14表达的降低可能导致OsIAA14蛋白的积累减少，作为调节生长素信号的机制。因此，我们的发现揭示了OsMADS25至少部分地通过基于AUX/IAA的生长素信号来调控水稻根系生长发育的分子机制。
The phytohormone auxin is essential for root development in plants. OsMADS25 is a homologue of the AGL17-clade MADS-box genes in rice. Despite recent progress, the molecular mechanisms underlying the regulation of root development by OsMADS25 are not well known. It is unclear whether OsMADS25 regulates root development via auxin signalling. In this study, we examined the role of OsMADS25 in root development and characterized the signalling pathway through which OsMADS25 regulates root system development in rice. OsMADS25 overexpression significantly increased, but RNAi gene silencing repressed primary root (PR) length and lateral root (LR) density. Moreover, OsMADS25 promoted LR development in response to NO3- . Further study showed that OsMADS25 increased auxin accumulation in the root system by enhancing auxin biosynthesis and transport, while also reducing auxin degradation, therefore stimulating root development. More importantly, OsMADS25 was found to regulate OsIAA14 expression directly by binding to the CArG-box in the promoter region of OsIAA14, which encodes an Aux/indole acetic acid (IAA) transcriptional repressor of auxin signalling. Elevated auxin levels and decreased OsIAA14 expression might lead to reduced OsIAA14 protein accumulation, as a mechanism to regulate auxin signalling. Therefore, our findings reveal a molecular mechanism by which OsMADS25 modulates root system growth and development in rice, at least partilly, via Aux/IAA-based auxin signalling.
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影响因子：
7.2
作者：
Ni Jun;Wang Gaohang;Wu Ping
通讯作者：
Wu Ping
影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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