Measurement of changes in endogenous serotonin level by positron emission tomography with [18F]altanserin
Measurement of changes in endogenous serotonin level by positron emission tomography with [18F]altanserin
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[18F]阿坦色林通过正电子发射断层扫描测量内源性血清素水平的变化
DOI：
10.1007/s12149-021-01633-4
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发表时间：
2021
影响因子：
2.6
通讯作者：
Suhara Tetsuya
中科院分区：
文献类型：
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作者：
Ikoma Yoko;Takuwa Hiroyuki;Nishino Asuka;Maeda Jun;Kawamura Kazunori;Obata Takayuki;Zhang Ming-Rong;Higuchi Makoto;Suhara Tetsuya
正电子发射断层扫描（PET）已被用于研究内源性神经递质浓度的变化。最近，该技术已被应用于使用[18F]阿坦色林的阿坦色林2A受体的成像。在这些测量中，结合电位（BP）的降低表明药理学或认知刺激引起的内源性5-羟色胺水平升高，BP降低的敏感性取决于[18 F]阿坦色林的特性。在这项研究中，我们评估了一种分析方法，用于估计内源性血清素水平的变化，该方法基于在基线和刺激状态下使用[18 F]阿坦色林的PET扫描，并使用模拟和小动物PET研究对其进行了验证。时间-使用扩展隔室模型产生基线和刺激状态下的活性曲线，所述扩展隔室模型包括细胞之间对受体的竞争。施用[18F]阿坦色林和内源性5-羟色胺。在刺激状态下，内源性5-羟色胺升高的幅度和发作发生不同程度的改变。在这些时间-活动曲线中，使用简化的参考组织模型（SRTM）估计BP，并通过与基线状态比较来评估BP的降低。接下来，将所提出的方法应用于小鼠PET研究。内源性5-羟色胺水平升高的治疗与选择性5-羟色胺再摄取抑制剂（SSRIs），PET研究进行了两次，一次与一次没有治疗。在这两个扫描，BP估计使用SRTM与小脑作为参考区域，并减少在BP SSRI治疗后进行了评估。ResultsIn的模拟，BP估计的刺激状态小于基线状态，和他们的减少有关的5-羟色胺浓度的变化量。血压降低也受到5-羟色胺升高的影响。在小鼠的研究中，大脑皮层的BP下降，在扫描与SSRI treatment.ConclusionsThe减少BP估计使用SRTM从[18 F]altanserin-PET研究在基线和刺激状态下可以检测到的变化，在结合条件的阿托宁2A受体。这可能有助于研究刺激引起的内源性5-羟色胺水平升高。
ObjectivePositron emission tomography (PET) has been used to investigate changes in the concentration of endogenous neurotransmitters. Recently, this technique has been applied to the imaging of serotonin2Areceptors using [18F]altanserin. In these measurements, a reduction in binding potential (BP) suggests an increase in endogenous serotonin levels caused by pharmacological or cognitive stimulations, and the sensitivity of BP reduction depends on the characteristics of [18F]altanserin. In this study, we evaluated an analytical method for estimating the changes in endogenous serotonin levels based on PET scans with [18F]altanserin at baseline and stimulated states and validated it using simulations and small animal PET studies.MethodsFirst, in the simulations, the time-activity curves at baseline and the stimulated states were generated using an extended compartment model including the competition for the receptors between the administered [18F]altanserin and endogenous serotonin. In the stimulated state, the magnitude and onset of the endogenous serotonin elevation were altered to varying degrees. In these time-activity curves, BP was estimated using the simplified reference tissue model (SRTM), and the reduction in BP was evaluated by comparison with that of the baseline state. Next, the proposed method was applied to mouse PET studies. Endogenous serotonin levels were elevated by treatment with selective serotonin reuptake inhibitors (SSRIs), and PET studies were performed twice, once with and once without treatment. In both scans, BP was estimated using the SRTM with the cerebellum as a reference region, and the reduction in BP after SSRI treatment was evaluated.ResultsIn the simulations, the BP estimate of the stimulated state was smaller than that of the baseline state, and their reduction was related to the amount of change in the serotonin concentration. BP reduction was also affected by the onset of serotonin elevation. In the mouse studies, the BP of the cerebral cortex decreased in the scans with SSRI treatment.ConclusionsThe reduction in BP estimated using the SRTM from [18F]altanserin-PET studies at baseline and in stimulated states can detect changes in the binding conditions of serotonin2Areceptors. This may be useful for investigating the elevation of endogenous serotonin levels caused by stimulations.
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5-HT2A 受体拮抗剂阿坦色林和 MDL 100907 的结合特性
影响因子：
2.3
作者：
H. Kristiansen;B. Elfving;P. Plenge;L. Pinborg;N. Gillings;G. Knudsen
通讯作者：
G. Knudsen
眶额皮质中认知诱导的血清素调节：使用 5-HT2a 受体拮抗剂 [18F]阿坦色林进行延迟匹配样本任务的受控交叉 PET 研究
影响因子：
5.7
作者：
H. Hautzel;H. Müller;H. Herzog;R. Grandt
通讯作者：
R. Grandt
新型 5-羟色胺 1A 受体拮抗剂 [3H]WAY-100635 与大鼠大脑结合的表征
影响因子：
4.7
作者：
X. Khawaja;N. Evans;Y. Reilly;C. Ennis;M. Minchin
通讯作者：
M. Minchin
使用右芬氟拉明激发和[18F]阿坦色林正电子发射断层扫描评估人脑中的血清素释放能力
影响因子：
5.7
作者：
B. Quednow;V. Treyer;F. Hasler;N. Dörig;M. Wyss;C. Burger;K. Rentsch;G. Westera;P. Schubiger;A. Buck;F. Vollenweider
通讯作者：
F. Vollenweider
Maeda, J.：“[羰基-11C] WAY-100635 的体内结合特性：内源性血清素的作用”突触。
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