Cell wall composition throughout development for the model grass Brachypodium distachyon.
Cell wall composition throughout development for the model grass Brachypodium distachyon.
复制标题
DOI：
10.3389/fpls.2012.00266
复制
发表时间：
2012
影响因子：
5.6
通讯作者：
Hatfield RD
中科院分区：
文献类型：
--
作者：
Rancour DM;Marita JM;Hatfield RD
温带多年生牧草是世界范围内重要的牲畜营养能源，也是生产木质纤维生物燃料的潜在原料。一年生温带牧草Brachypodium disachyon一直被认为是一个有用的模式系统，有助于基于系统发育关系对农业上重要的温带牧草进行生物学研究。为了在物理上证实遗传预测，我们测定了两个常见的二倍体青霉Bd21-3和Bd21的发育过程中器官特异细胞壁的化学成分分布。从不同器官(叶、鞘、茎和根)分离的细胞壁在(1)12天幼苗、(2)营养到生殖过渡和(3)成熟种子灌浆的三个发育阶段进行了化学分析。此外，我们还包括用于遗传转化的胚性愈伤组织的细胞壁分析。由木质素、羟基肉桂酸酯、糖醛酸基、中性糖和蛋白质组成的细胞壁的组成表明，远缘短柄草在化学上与农业上重要的饲草相似。Bd21-3和Bd21的羟基肉桂酸组成略有差异。此外，与农艺上重要的C3草相比，更成熟的短柄蕨干细胞壁的葡萄糖相对增加了48%，木质素减少了36%。虽然Brachypodium与农学上重要的C3草之间存在差异，但Brachypodium disachyon仍然是一个有用的遗传操作细胞壁组成的模式系统，以确定其对瘤胃消化率或生物能生产的能量转换效率等功能特性的影响。
Temperate perennial grasses are important worldwide as a livestock nutritive energy source and a potential feedstock for lignocellulosic biofuel production. The annual temperate grass Brachypodium distachyon has been championed as a useful model system to facilitate biological research in agriculturally important temperate forage grasses based on phylogenetic relationships. To physically corroborate genetic predictions, we determined the chemical composition profiles of organ-specific cell walls throughout the development of two common diploid accessions of Brachypodium distachyon, Bd21-3 and Bd21. Chemical analysis was performed on cell walls isolated from distinct organs (i.e., leaves, sheaths, stems, and roots) at three developmental stages of (1) 12-day seedling, (2) vegetative-to-reproductive transition, and (3) mature seed fill. In addition, we have included cell wall analysis of embryonic callus used for genetic transformations. Composition of cell walls based on components lignin, hydroxycinnamates, uronosyls, neutral sugars, and protein suggests that Brachypodium distachyon is similar chemically to agriculturally important forage grasses. There were modest compositional differences in hydroxycinnamate profiles between accessions Bd21-3 and Bd21. In addition, when compared to agronomical important C3 grasses, more mature Brachypodium stem cell walls have a relative increase in glucose of 48% and a decrease in lignin of 36%. Though differences exist between Brachypodium and agronomical important C3 grasses, Brachypodium distachyon should be still a useful model system for genetic manipulation of cell wall composition to determine the impact upon functional characteristics such as rumen digestibility or energy conversion efficiency for bioenergy production.
登录
查看更多内容
影响因子：
3.8
作者：
GRABBER, JH;HATFIELD, RD;AMRHEIN, N
通讯作者：
AMRHEIN, N
影响因子：
6.9
作者：
Guillon, Fabienne;Bouchet, Brigitte;Saulnier, Luc
通讯作者：
Saulnier, Luc
影响因子：
3.8
作者：
Christensen, Ulla;Alonso-Simon, Ana;Harholt, Jesper
通讯作者：
Harholt, Jesper
影响因子：
6.9
作者：
Guillon F;Larré C;Petipas F;Berger A;Moussawi J;Rogniaux H;Santoni A;Saulnier L;Jamme F;Miquel M;Lepiniec L;Dubreucq B
通讯作者：
Dubreucq B
影响因子：
6.2
作者：
Christiansen, P;Andersen, CH;Nielsen, KK
通讯作者：
Nielsen, KK