Expression of SPANX proteins in normal prostatic tissue and in prostate cancer.
Expression of SPANX proteins in normal prostatic tissue and in prostate cancer.
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DOI：
10.4081/ejh.2010.e41
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Rappazzo G
中科院分区：
文献类型：
--
作者：
Salemi M;Calogero AE;Zaccarello G;Castiglione R;Cosentino A;Campagna C;Vicari E;Rappazzo G
与X染色体核相关的精子蛋白(SPANX)基因家族编码的蛋白质不仅在生殖细胞中表达，而且在许多肿瘤中也表达。此外，SPANX基因定位在X染色体的一个区间(即Xq27)，通过连锁分析发现这与家族性前列腺癌有关。因此，这项研究的目的是评估SPANX蛋白在正常前列腺组织和前列腺癌中的表达。为此，对15例正常(尸检)供者和12例前列腺癌患者的福尔马林固定和石蜡包埋切片进行免疫组织化学分析。大约40%的正常和肿瘤前列腺样本SPANX呈阳性。在正常前列腺细胞中，信号仅在细胞核内，而在肿瘤细胞中，核阳性和胞浆阳性均可观察到。综上所述，这些发现表明，SPANX基因在正常前列腺和肿瘤前列腺中都有表达，但后者表现出特殊的胞浆染色阳性。这表明SPANX基因的过度表达可能与前列腺癌的发生有关。还需要更多的研究来证实这一假设。
The sperm protein associated with the nucleus in the X chromosome (SPANX) gene family encodes for proteins that are not only expressed in germ cells, but also in a number of tumors. In addition, SPANX genes map in an interval of the X chromosome (namely, Xq27), which has been found to be associated with familial prostate cancer by linkage analysis. The aim of this study was therefore to evaluate SPANX protein expression in normal prostate tissues and in prostate carcinoma. For this purpose, formalin-fixed and paraffin-embedded sections obtained from 15 normal (at autopsy) donors and 12 men with prostate cancer were analyzed by immunohistochemistry. About 40% of both normal and tumor prostate samples resulted SPANX positive. Signals were exclusively within the nucleus in normal prostate cells, whereas both nuclear and cytoplasmic positivity was observed in tumor cells. In conclusion, these findings showed that SPANX genes are expressed in both normal and tumor prostate gland, but the latter showed a peculiar cytoplasmic staining positivity. This suggests a possible association between SPANX over expression and prostate cancer development. Additional studies are needed to corroborate this hypothesis.
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影响因子：
5.3
作者：
Baffoe-Bonnie, AB;Smith, JB;Bailey-Wilson, JE
通讯作者：
Bailey-Wilson, JE
影响因子：
3.6
作者：
Westbrook, VA;Diekman, AB;Herr, JC
通讯作者：
Herr, JC
影响因子：
56.9
作者：
Bailey, JA;Gu, ZP;Eichler, EE
通讯作者：
Eichler, EE
影响因子：
--
作者：
Salemi, M;Calogero, AE;Vicari, E
通讯作者：
Vicari, E
影响因子：
1.1
作者：
Salemi, Michele;Calogero, Aldo E.;Rappazzo, Giancarlo
通讯作者：
Rappazzo, Giancarlo