Tunable chemokine production by antigen presenting dendritic cells in response to changes in regulatory T cell frequency in mouse reactive lymph nodes.
Tunable chemokine production by antigen presenting dendritic cells in response to changes in regulatory T cell frequency in mouse reactive lymph nodes.
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DOI：
10.1371/journal.pone.0007696
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发表时间：
2009-11-06
期刊：
影响因子：
3.7
通讯作者：
Sarukhan A
中科院分区：
文献类型：
--
作者：
Dal Secco V;Soldani C;Debrat C;Asperti-Boursin F;Donnadieu E;Viola A;Sarukhan A
尽管有证据表明调节性 T 细胞 (Treg) 可以抑制免疫反应的效应期，但很明显，它们的主要作用是抑制次级淋巴器官中的 T 细胞启动。最近使用两个光子激光显微镜进行的实验表明，树突状细胞 (DC) 是 Treg 细胞功能的核心，并且 T 细胞调节的体内机制比体外描述的机制更为复杂。在这里，我们试图确定 Treg 数量的调节是否以及如何改变淋巴结 (LN) 微环境。我们发现促炎趋化因子——CCL2（MCP-1）和CCL3（MIP-1a）——在T细胞激活后早期（24小时）在LN中分泌，这种分泌依赖于抗原特异性DC-T细胞相互作用，并且与对相同抗原具有特异性的Tregs的频率成反比。此外，我们证明Tregs改变了抗原呈递DC的化学引诱特性，随着Tregs频率的增加，Tregs无法产生CCL2和CCL3并吸引抗原特异性T细胞。这些结果证实了 Tregs 在抗原特异性免疫反应期间 LN 模式形成中的主要作用。
Although evidence exists that regulatory T cells (Tregs) can suppress the effector phase of immune responses, it is clear that their major role is in suppressing T cell priming in secondary lymphoid organs. Recent experiments using two photon laser microscopy indicate that dendritic cells (DCs) are central to Treg cell function and that the in vivo mechanisms of T cell regulation are more complex than those described in vitro. Here we have sought to determine whether and how modulation of Treg numbers modifies the lymph node (LN) microenvironment. We found that pro-inflammatory chemokines—CCL2 (MCP-1) and CCL3 (MIP-la)—are secreted in the LN early (24 h) after T cell activation, that this secretion is dependent on antigen-specific DC–T cell interactions, and that it was inversely related to the frequency of Tregs specific for the same antigen. Furthermore, we demonstrate that Tregs modify the chemoattractant properties of antigen-presenting DCs, which, as the frequency of Tregs increases, fail to produce CCL2 and CCL3 and to attract antigen-specific T cells. These results substantiate a major role of Tregs in LN patterning during antigen-specific immune responses.
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影响因子：
32.4
作者：
Hill, Jonathan A.;Feuerer, Markus;Benoist, Christophe
通讯作者：
Benoist, Christophe
影响因子：
30.5
作者：
Hugues, Stephanie;Scholer, Alix;Fetler, Luc
通讯作者：
Fetler, Luc
DOI：
10.1084/jem.192.10.1425
发表时间：
2000-11-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gretz JE;Norbury CC;Anderson AO;Proudfoot AE;Shaw S
通讯作者：
Shaw S
影响因子：
4.4
作者：
Castellino, Flora;Germain, Ronald N.
通讯作者：
Germain, Ronald N.
影响因子：
15.3
作者：
Dieu, M C;Vanbervliet, B;Vicari, A;Bridon, J M;Oldham, E;Ait-Yahia, S;Briere, F;Zlotnik, A;Lebecque, S;Caux, C
通讯作者：
Caux, C