Lymph-borne chemokines and other low molecular weight molecules reach high endothelial venules via specialized conduits while a functional barrier limits access to the lymphocyte microenvironments in lymph node cortex.
Lymph-borne chemokines and other low molecular weight molecules reach high endothelial venules via specialized conduits while a functional barrier limits access to the lymphocyte microenvironments in lymph node cortex.
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DOI：
10.1084/jem.192.10.1425
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发表时间：
2000-11-20
期刊：
影响因子：
--
通讯作者：
Shaw S
中科院分区：
文献类型：
--
作者：
Gretz JE;Norbury CC;Anderson AO;Proudfoot AE;Shaw S
淋巴携带的可溶性因子（例如，趋化因子等）影响淋巴结皮质中高内皮微静脉（HEVs）处的淋巴细胞再循环和内皮表型。然而，淋巴携带的可溶性分子从包膜下窦到HEV的途径尚不清楚。因此，我们将各种荧光团标记的可溶性分子皮下注射到小鼠和大鼠中，并检查它们在引流淋巴结中的分布。所有分子，包括微生物脂多糖，在被膜下和髓窦中非常明显，而不是在整个引流淋巴结中扩散，但在很大程度上被排除在皮质淋巴细胞微环境之外。即使在伴随病毒感染的急性淋巴结肿大期间也普遍存在排斥。然而，包括趋化因子在内的低分子量（MW）分子确实进入了皮质，但以非常明确的方式进入。低分子量，荧光标记的分子突出了囊下窦，网状纤维，以及相关的HEV的近腔和腔表面。这些低分子量分子位于网状网络的纤维中，网状网络是由成纤维细胞网状细胞包裹的胶原纤维网，通过与基底膜缠绕连接被膜下窦底和HEV。因此，低分子量，淋巴细胞携带的分子，包括趋化因子，迅速从包膜下窦到HEV使用的网状网络作为管道。
Lymph-borne, soluble factors (e.g., chemokines and others) influence lymphocyte recirculation and endothelial phenotype at high endothelial venules (HEVs) in lymph node cortex. Yet the route lymph-borne soluble molecules travel from the subcapsular sinus to the HEVs is unclear. Therefore, we injected subcutaneously into mice and rats a wide variety of fluorophore-labeled, soluble molecules and examined their distribution in the draining lymph nodes. Rather than percolating throughout the draining lymph node, all molecules, including microbial lipopolysaccharide, were very visible in the subcapsular and medullary sinuses but were largely excluded from the cortical lymphocyte microenvironments. Exclusion prevailed even during the acute lymph node enlargement accompanying viral infection. However, low molecular mass (MW) molecules, including chemokines, did gain entry into the cortex, but in a very defined manner. Low MW, fluorophore-labeled molecules highlighted the subcapsular sinus, the reticular fibers, and the abluminal and luminal surfaces of the associated HEVs. These low MW molecules were in the fibers of the reticular network, a meshwork of collagen fibers ensheathed by fibroblastic reticular cells that connects the subcapsular sinus floor and the HEVs by intertwining with their basement membranes. Thus, low MW, lymph-borne molecules, including chemokines, traveled rapidly from the subcapsular sinus to the HEVs using the reticular network as a conduit.
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影响因子：
--
作者：
COMPTON, CC;RAVIOLA, E
通讯作者：
RAVIOLA, E
影响因子：
--
作者：
HAYAKAWA, M;KOBAYASHI, M;HOSHINO, T
通讯作者：
HOSHINO, T
DOI：
10.1111/j.1749-6632.1996.tb52953.x
发表时间：
1996-01-01
期刊：
MICROBIAL PATHOGENESIS AND IMMUNE RESPONSE II
影响因子：
--
作者：
Ebnet, K;Simon, MM;Shaw, S
通讯作者：
Shaw, S
影响因子：
7.8
作者：
Anderson, A O;Shaw, S
通讯作者：
Shaw, S
影响因子：
--
作者：
FARR, AG;CHO, Y;DEBRUYN, PPH
通讯作者：
DEBRUYN, PPH