Multifaceted investigation of metabolites during nitrogen fixation in Medicago via high resolution MALDI-MS imaging and ESI-MS.
Multifaceted investigation of metabolites during nitrogen fixation in Medicago via high resolution MALDI-MS imaging and ESI-MS.
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DOI：
10.1007/s13361-014-1010-0
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发表时间：
2015-01
影响因子：
3.2
通讯作者：
Li, Lingjun
中科院分区：
文献类型：
--
作者：
Gemperline, Erin;Jayaraman, Dhileepkumar;Maeda, Junko;Ane, Jean-Michel;Li, Lingjun
豆类已经发展出与土壤细菌建立共生关系的独特能力，这种关系被称为根瘤菌。这种相互作用导致根瘤的形成，根瘤菌在根瘤中茁壮成长，并通过生物固氮(BNF)将大气中的氮素还原为植物可用的铵。由于两个共生伙伴的遗传信息的可用性，紫花苜蓿-紫花苜蓿根瘤菌联合是研究生物固氮过程的一个很好的模型。虽然代谢物在这种共生关系中很重要，但很少有研究调查影响这一过程的代谢物阵列。在这些研究中，大多数只针对少数特定的代谢物，这些代谢物的作用要么是众所周知的，要么是特征明确的代谢途径的一部分。在这里，我们使用了多方面的质谱学(MS)方法来检测和鉴定生物固氮过程中存在的关键代谢物，并以紫花苜蓿-紫花苜蓿中华根瘤菌联合为模型系统。在这项研究中使用了高质量精度和高分辨率的基质辅助激光解吸/电离(MALDI)和电喷雾电离(ESI)轨道仪器，为更深入地表征固氮过程提供了补充结果。我们使用了特性良好的植物和细菌突变体来强调功能性和非功能性结节中存在的代谢物之间的差异。我们的研究强调了结合使用质谱学技术来检测代谢物组成的差异和这些代谢物在植物生物学中的分布的好处。
Legumes have developed the unique ability to establish a symbiotic relationship with soil bacteria known as rhizobia. This interaction results in the formation of root nodules in which rhizobia thrive and reduce atmospheric dinitrogen into plant-usable ammonium through biological nitrogen fixation (BNF). Due to the availability of genetic information for both of the symbiotic partners, the Medicago truncatula–Sinorhizobium meliloti association is an excellent model for examining the BNF process. Although metabolites are important in this symbiotic association, few studies have investigated the array of metabolites that influence this process. Of these studies, most target only a few specific metabolites, the roles of which are either well known or are part of a well-characterized metabolic pathway. Here, we used a multifaceted mass spectrometric (MS) approach to detect and identify the key metabolites that are present during BNF using the Medicago truncatula–Sinorhizobium meliloti association as the model system. High mass accuracy and high resolution matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) Orbitrap instruments were used in this study and provide complementary results for more in-depth characterization of the nitrogen-fixation process. We used well-characterized plant and bacterial mutants to highlight differences between the metabolites that are present in functional vs. non-functional nodules. Our study highlights the benefits of using a combination of mass spectrometric techniques to detect differences in metabolite composition and the distributions of these metabolites in plant biology.
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影响因子：
4.3
作者：
Fischinger, Stephanie A.;Drevon, Jean-Jacques;Schulze, Joachim
通讯作者：
Schulze, Joachim
影响因子：
1.2
作者：
Gemperline, Erin;Li, Lingjun
通讯作者：
Li, Lingjun
影响因子：
7.4
作者：
Mitra, RM;Long, SR
通讯作者：
Long, SR
影响因子：
62.1
作者：
Hoffman, Brian M.;Lukoyanov, Dmitriy;Yang, Zhi-Yong;Dean, Dennis R.;Seefeldt, Lance C.
通讯作者：
Seefeldt, Lance C.
影响因子：
7.4
作者：
Graham, PH;Vance, CP
通讯作者：
Vance, CP