Improving Release of Liposome-Encapsulated Drugs with Focused Ultrasound and Vaporizable Droplet-Liposome Nanoclusters.
Improving Release of Liposome-Encapsulated Drugs with Focused Ultrasound and Vaporizable Droplet-Liposome Nanoclusters.
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DOI：
10.3390/pharmaceutics13050609
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发表时间：
2021-04-22
期刊：
影响因子：
5.4
通讯作者：
Sirsi SR
中科院分区：
文献类型：
--
作者：
Honari A;Merillat DA;Bellary A;Ghaderi M;Sirsi SR
小分子药物的主动靶向递送在个性化治疗中变得越来越重要，特别是在癌症、脑部疾病和各种其他疾病中。然而，目前仍然缺乏有效的空间靶向和在体内递送高药物有效载荷的手段。聚焦超声结合过热的相移纳米液滴，利用热量和声音蒸发成微泡，正迅速成为一种流行的靶向药物递送策略。聚焦超声在不使用电离能的情况下，以极好的空间精度瞄准深层组织，从而激活循环中的纳米液滴。该技术的主要限制之一是药物在液滴核心或外壳材料中的装载能力差。为了满足这一需求，我们开发了一种策略，将低沸点十氟丁烷和八氟丙烷（DFB和OFP）纳米液滴与载药脂质体结合起来，形成可相变的液滴-脂质体簇（pdlc）。我们展示了一种在液滴表面组装具有高载药能力的亚微米pdlc的简便方法。此外，我们证明了pdlc中脂质体的化学系缚可以在声激活时快速释放其封装的货物（使用基于ofp的pdlc时为60%）。小分子药物的快速解包裹将使其在靶组织中立即生物利用，或在血管内释放后促进其在局部组织中的更好渗透。本研究中开发的pdlc可用于递送多种脂质体封装疗法或显像剂，用于多模态成像应用。我们还概述了一种使用聚焦超声能量和pdlc将替代包膜药物荧光素输送到体内肿瘤的策略。
Active targeted delivery of small molecule drugs is becoming increasingly important in personalized therapies, especially in cancer, brain disorders, and a wide variety of other diseases. However, effective means of spatial targeting and delivering high drug payloads in vivo are still lacking. Focused ultrasound combined with superheated phase-shift nanodroplets, which vaporize into microbubbles using heat and sound, are rapidly becoming a popular strategy for targeted drug delivery. Focused ultrasound can target deep tissue with excellent spatial precision and without using ionizing energy, thus can activate nanodroplets in circulation. One of the main limitations of this technology has been poor drug loading in the droplet core or the shell material. To address this need, we have developed a strategy to combine low-boiling point decafluorabutane and octafluoropropane (DFB and OFP) nanodroplets with drug-loaded liposomes, creating phase-changeable droplet-liposome clusters (PDLCs). We demonstrate a facile method of assembling submicron PDLCs with high drug-loading capacity on the droplet surface. Furthermore, we demonstrate that chemical tethering of liposomes in PDLCs enables a rapid release of their encapsulated cargo upon acoustic activation (>60% using OFP-based PDLCs). Rapid uncaging of small molecule drugs would make them immediately bioavailable in target tissue or promote better penetration in local tissue following intravascular release. PDLCs developed in this study can be used to deliver a wide variety of liposome-encapsulated therapeutics or imaging agents for multi-modal imaging applications. We also outline a strategy to deliver a surrogate encapsulated drug, fluorescein, to tumors in vivo using focused ultrasound energy and PDLCs.
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影响因子：
10.8
作者：
Airan RD;Meyer RA;Ellens NP;Rhodes KR;Farahani K;Pomper MG;Kadam SD;Green JJ
通讯作者：
Green JJ
DOI：
10.1016/s0360-3016(96)00389-6
发表时间：
1996-12-01
影响因子：
7
作者：
Gaber, MH;Wu, NZ;Papahadjopoulos, D
通讯作者：
Papahadjopoulos, D
影响因子：
2.9
作者：
Burgess MT;Porter TM
通讯作者：
Porter TM
对快速，敏感，声学生物传感的过热纳米圆柱的选择性蒸发。
影响因子：
10
作者：
Chattaraj R;Mohan P;Besmer JD;Goodwin AP
通讯作者：
Goodwin AP
影响因子：
3.7
作者：
Koning, Gerben A.;Eggermont, Alexander M. M.;Lindner, Lars H.;ten Hagen, Timo L. M.
通讯作者：
ten Hagen, Timo L. M.