The enhancement of tolerance to salt and cold stresses by modifying the redox state and salicylic acid content via the cytosolic malate dehydrogenase gene in transgenic apple plants.
The enhancement of tolerance to salt and cold stresses by modifying the redox state and salicylic acid content via the cytosolic malate dehydrogenase gene in transgenic apple plants.
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DOI：
10.1111/pbi.12556
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发表时间：
2016-10
影响因子：
13.8
通讯作者：
Yao YX
中科院分区：
文献类型：
--
作者：
Wang QJ;Sun H;Dong QL;Sun TY;Jin ZX;Hao YJ;Yao YX
本研究以苹果胞质苹果酸脱氢酶基因（MdcyMDH）为研究对象，分析了其在苹果耐盐性和耐冷性中的作用，并探讨了其在苹果耐盐性和耐冷性中的调控机制。MdcyMDH转录物由轻度冷和盐处理诱导，并且与野生型（WT）植物相比，MdcyMDH过表达的苹果植物具有改善的耐冷性和耐盐性。数字基因表达标签分析表明，MdcyMDH的过度表达在很大程度上改变了一些生物学过程，包括激素信号转导，光合作用，柠檬酸循环和氧化还原。进一步的实验证实，MdcyMDH过表达改变了线粒体和叶绿体代谢，并提高了还原力的水平，主要是由增加的抗坏血酸和谷胱甘肽，以及增加的抗坏血酸/脱氢抗坏血酸和谷胱甘肽/谷胱甘肽二硫化物的比例，在正常和特别是应激条件下。同时，与WT植物相比，转基因植物产生高的H2 O2含量，但观察到低的生产率。另一方面，在正常和胁迫条件下，转基因植物比WT植物积累更多的游离和总水杨酸（SA）。总之，MdcyMDH通过产生更多的还原性氧化还原态和增加SA水平赋予转基因苹果植物更高的胁迫耐受性; MdcyMDH可以作为基因工程耐盐和耐冷树木的靶基因。
In this study, we characterized the role of an apple cytosolic malate dehydrogenase gene (MdcyMDH) in the tolerance to salt and cold stresses and investigated its regulation mechanism in stress tolerance. The MdcyMDH transcript was induced by mild cold and salt treatments, and MdcyMDH‐overexpressing apple plants possessed improved cold and salt tolerance compared to wild‐type (WT) plants. A digital gene expression tag profiling analysis revealed that MdcyMDH overexpression largely altered some biological processes, including hormone signal transduction, photosynthesis, citrate cycle and oxidation–reduction. Further experiments verified that MdcyMDH overexpression modified the mitochondrial and chloroplast metabolisms and elevated the level of reducing power, primarily caused by increased ascorbate and glutathione, as well as the increased ratios of ascorbate/dehydroascorbate and glutathione/glutathione disulphide, under normal and especially stress conditions. Concurrently, the transgenic plants produced a high H2O2 content, but a low production rate was observed compared to the WT plants. On the other hand, the transgenic plants accumulated more free and total salicylic acid (SA) than the WT plants under normal and stress conditions. Taken together, MdcyMDH conferred the transgenic apple plants a higher stress tolerance by producing more reductive redox states and increasing the SA level; MdcyMDH could serve as a target gene to genetically engineer salt‐ and cold‐tolerant trees.
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影响因子：
5.3
作者：
Feng XM;Zhao Q;Zhao LL;Qiao Y;Xie XB;Li HF;Yao YX;You CX;Hao YJ
通讯作者：
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作者：
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作者：
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影响因子：
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作者：
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