The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1 encodes an ICE-like protein in apple.
The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1 encodes an ICE-like protein in apple.
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DOI：
10.1186/1471-2229-12-22
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发表时间：
2012-02-15
影响因子：
5.3
通讯作者：
Hao YJ
中科院分区：
文献类型：
--
作者：
Feng XM;Zhao Q;Zhao LL;Qiao Y;Xie XB;Li HF;Yao YX;You CX;Hao YJ
植物生长受低温影响很大，低温胁迫诱导了许多基因的表达。虽然在拟南芥中已经发现了许多与冷胁迫相关的基因，但对苹果中参与冷胁迫反应的转录因子却知之甚少。在这里，我们表明，苹果bHLH（基本螺旋环螺旋）基因MdCIbHLH 1（冷诱导bHLH 1），它编码的ICE样蛋白，显着诱导响应冷胁迫。MdCibHLH 1蛋白特异性结合AtCBF 3启动子中的MYC识别序列，并且MdCibHLH 1过表达增强转基因拟南芥的耐冷性。此外，MdCIbHLH 1蛋白结合到MdCBF 2的启动子上，并通过CBF（C-repeat-binding factor）途径上调MdCBF 2的表达，从而有利于转基因苹果的耐冷性。我们的研究结果表明，MdCibHLH 1功能在不同物种的胁迫耐受性。例如，异位MdCibHLH 1表达赋予转基因烟草增强的耐冷性。最后，我们观察到，冷诱导苹果MdCIbHLH 1蛋白的降解，这种降解可能是由泛素化和sumoylation介导的。基于这些发现，MdCIbHLH 1编码一个转录因子，该因子对苹果的耐冷反应非常重要。
Plant growth is greatly affected by low temperatures, and the expression of a number of genes is induced by cold stress. Although many genes in the cold signaling pathway have been identified in Arabidopsis, little is known about the transcription factors involved in the cold stress response in apple. Here, we show that the apple bHLH (basic helix-loop-helix) gene MdCIbHLH1 (Cold-Induced bHLH1), which encodes an ICE-like protein, was noticeably induced in response to cold stress. The MdCIbHLH1 protein specifically bound to the MYC recognition sequences in the AtCBF3 promoter, and MdCIbHLH1 overexpression enhanced cold tolerance in transgenic Arabidopsis. In addition, the MdCIbHLH1 protein bound to the promoters of MdCBF2 and favorably contributed to cold tolerance in transgenic apple plants by upregulating the expression of MdCBF2 through the CBF (C-repeat-binding factor) pathway. Our findings indicate that MdCIbHLH1 functions in stress tolerance in different species. For example, ectopic MdCIbHLH1 expression conferred enhanced chilling tolerance in transgenic tobacco. Finally, we observed that cold induces the degradation of the MdCIbHLH1 protein in apple and that this degradation was potentially mediated by ubiquitination and sumoylation. Based on these findings, MdCIbHLH1 encodes a transcription factor that is important for the cold tolerance response in apple.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
7.4
作者：
Jaglo, KR;Kleff, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K