Extracellular calcium differentially affects intracellular responses to nanosecond pulsed electric fields
Extracellular calcium differentially affects intracellular responses to nanosecond pulsed electric fields
复制标题
细胞外钙对细胞内对纳秒脉冲电场的反应有不同的影响
DOI：
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Ken
中科院分区：
文献类型：
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作者：
Keiko Morotomi‐Yano;Ken
纳秒脉冲电场（nsPEFs）由于其对活细胞的独特作用而受到相当大的关注。已知将培养的细胞暴露于nsPEF通过在质膜中形成小孔来诱导Ca内流。此外，据报道，nsPEFs可诱导多种细胞信号传导途径的激活。为了研究nsPEF暴露后Ca内流和细胞内信号事件之间的关系，我们将nsPEF应用于悬浮在无Ca或含Ca培养基中的HeLa S3细胞，并分析信号通路的激活。我们观察到细胞外Ca的存在是AMPK激活所必需的，但对于nsPEF暴露的HeLa S3细胞中的应激反应的起始是必需的。JNK和p38是MAPK通路中的主要调节因子，它们被nsPEFs激活，而不管细胞外Ca的存在与否。然而，细胞外Ca的缺乏导致JNK和p38的磷酸化增强，这表明Ca内流对MAPK通路中的负反馈控制具有积极作用。综上所述，这些观察结果表明，由nsPEFs诱导的Ca内流差异参与细胞内信号传导事件。
Nanosecond pulsed electric fields (nsPEFs) have received considerable attention because of their unique effects on living cells. Exposure of cultured cells to nsPEFs is known to induce Ca influx by forming small pores in the plasma membrane. Furthermore, nsPEFs have been reported to induce activation of multiple cellular signaling pathways. To examine the relationship between Ca influx and intracellular signaling events after nsPEF exposure, we applied nsPEFs to HeLa S3 cells suspended in either Ca-free or Ca-containing medium and analyzed the activation of signaling pathways. We observed that the presence of extracellular Ca was required for AMPK activation but dispensable for the initiation of stress responses in nsPEF-exposed HeLa S3 cells. JNK and p38, which are major regulatory factors in MAPK pathways, were activated by nsPEFs irrespective of the presence or absence of extracellular Ca. The absence of extracellular Ca, however, led to augmented phosphorylation of JNK and p38, suggesting positive effects of Ca influx on negative feedback controls in MAPK pathways. Taken together, these observations demonstrate that Ca influx induced by nsPEFs differentially participates in intracellular signaling events.
影响因子：
11.4
作者：
Gupta, S;Barrett, T;Davis, RJ
通讯作者：
Davis, RJ
DOI：
10.1016/j.bbrc.2009.05.035
发表时间：
2009-07-24
影响因子：
3.1
作者：
Pakhomov, Andrei G.;Bowman, Angela M.;Ibey, Bennett L.;Andre, Franck M.;Pakhomova, Olga N.;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.