Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane.
Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane.
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DOI：
10.1016/j.bbrc.2009.05.035
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发表时间：
2009-07-24
影响因子：
3.1
通讯作者：
Schoenbach, Karl H.
中科院分区：
文献类型：
--
作者：
Pakhomov, Andrei G.;Bowman, Angela M.;Ibey, Bennett L.;Andre, Franck M.;Pakhomova, Olga N.;Schoenbach, Karl H.
通过电脉冲（EP）或电穿孔的细胞透化已经被很好地确立为不加区别地增加水溶质沿浓度和电压梯度向下的膜流动的工具。然而，我们发现，纳秒持续时间（nsEPs）的EP触发电压敏感和内向整流膜孔的形成。NsEP处理的细胞大多数对丙啶保持不可渗透，表明最大孔径为~1 nm。如果nsEP打开的纳米孔破裂成更大的、可渗透丙啶的“常规”孔，则它们的离子通道样性质消失。然而，纳米孔可以稳定许多分钟，并显著影响电池电解质和水平衡。多个nsEP导致快速细胞肿胀和水泡，而用毛地黄皂苷打开较大的毛孔则消除肿胀并导致水泡内爆。通过磷脂酰丝氨酸残基的快速外化证实了nsEP打开的纳米孔的脂质性质。纳米孔构成了一个以前未开发的离子转运途径，补充经典的离子通道，但明显不同于它们。
Cell permeabilization by electric pulses (EPs), or electroporation, has been well established as a tool to indiscriminately increase membrane flows of water solutes down the concentration and voltage gradients. However, we found that EPs of nanosecond duration (nsEPs) trigger formation of voltage-sensitive and inward-rectifying membrane pores. NsEP-treated cells remain mostly impermeable to propidium, suggesting that the maximum pore size is ~1 nm. The ion-channel- like properties of nsEP-opened nanopores vanish if they break into larger, propidium-permeable “conventional” pores. However, nanopores can be stable for many minutes and significantly impact cell electrolyte and water balance. Multiple nsEPs cause fast cell swelling and blebbing, whereas opening of larger pores with digitonin abolishes swelling and causes blebs to implode. The lipid nature of nsEP-opened nanopores is confirmed by fast externalization of phosphatidylserine residues. Nanopores constitute a previously unexplored ion transport pathway that supplements classic ion channels but is distinctly different from them.
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影响因子：
--
作者：
Vernier, P. Thomas;Sun, Yinghua;Gundersen, Martin A.
通讯作者：
Gundersen, Martin A.
影响因子：
1.9
作者：
Pakhomov, Andrei G.;Kolb, Juergen F.;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
3.9
作者：
Pakhomov, Andrei G.;Shevin, Rachael;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
1.5
作者：
Weaver, JC
通讯作者：
Weaver, JC
影响因子：
4.4
作者：
Cervera, J;Schiedt, B;Ramírez, P
通讯作者：
Ramírez, P