Phenotypic Diagnosis of Lineage and Differentiation During Sake Yeast Breeding.
Phenotypic Diagnosis of Lineage and Differentiation During Sake Yeast Breeding.
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DOI：
10.1534/g3.117.044099
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发表时间：
2017-08-07
期刊：
影响因子：
--
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Ohnuki S;Okada H;Friedrich A;Kanno Y;Goshima T;Hasuda H;Inahashi M;Okazaki N;Tamura H;Nakamura R;Hirata D;Fukuda H;Shimoi H;Kitamoto K;Watanabe D;Schacherer J;Akao T;Ohya Y
清酒酵母是在日本独家开发的。它在繁殖过程中的多样性在很大程度上仍然是未知的。为了评估清酒谱系的育种过程，我们使用高维、单细胞、形态学表型分析彻底研究了27种清酒酵母菌株的表型和分化。虽然清酒酵母谱系的遗传多样性相对较低，但与整个酿酒酵母物种相比，其形态多样性已大幅扩大。不同类型的育种过程的评价表明，杂交一代（杂交育种）比突变体的分离（突变育种）对细胞形态有更深远的影响。表型稳健性分析显示，一些清酒酵母菌株在形态上更加异质，这可能是由于细胞网络枢纽受损所致。本研究为酵母育种遗传学和微生物育种策略的研究提供了新的视角。
Sake yeast was developed exclusively in Japan. Its diversification during breeding remains largely uncharacterized. To evaluate the breeding processes of the sake lineage, we thoroughly investigated the phenotypes and differentiation of 27 sake yeast strains using high-dimensional, single-cell, morphological phenotyping. Although the genetic diversity of the sake yeast lineage is relatively low, its morphological diversity has expanded substantially compared to that of the Saccharomyces cerevisiae species as a whole. Evaluation of the different types of breeding processes showed that the generation of hybrids (crossbreeding) has more profound effects on cell morphology than the isolation of mutants (mutation breeding). Analysis of phenotypic robustness revealed that some sake yeast strains are more morphologically heterogeneous, possibly due to impairment of cellular network hubs. This study provides a new perspective for studying yeast breeding genetics and micro-organism breeding strategies.
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影响因子：
4.4
作者：
Herrero-Medrano JM;Megens HJ;Groenen MA;Bosse M;Pérez-Enciso M;Crooijmans RP
通讯作者：
Crooijmans RP
DOI：
10.1093/dnares/dsr029
发表时间：
2011-12
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Akao T;Yashiro I;Hosoyama A;Kitagaki H;Horikawa H;Watanabe D;Akada R;Ando Y;Harashima S;Inoue T;Inoue Y;Kajiwara S;Kitamoto K;Kitamoto N;Kobayashi O;Kuhara S;Masubuchi T;Mizoguchi H;Nakao Y;Nakazato A;Namise M;Oba T;Ogata T;Ohta A;Sato M;Shibasaki S;Takatsume Y;Tanimoto S;Tsuboi H;Nishimura A;Yoda K;Ishikawa T;Iwashita K;Fujita N;Shimoi H
通讯作者：
Shimoi H
影响因子：
8.5
作者：
GAMEL, JW;AXELROD, DE
通讯作者：
AXELROD, DE
影响因子：
5.3
作者：
Bourguiba H;Audergon JM;Krichen L;Trifi-Farah N;Mamouni A;Trabelsi S;D'Onofrio C;Asma BM;Santoni S;Khadari B
通讯作者：
Khadari B
DOI：
10.1534/g3.113.007492
发表时间：
2013-12-09
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Cromie GA;Hyma KE;Ludlow CL;Garmendia-Torres C;Gilbert TL;May P;Huang AA;Dudley AM;Fay JC
通讯作者：
Fay JC