Loss of genetic diversity as a signature of apricot domestication and diffusion into the Mediterranean Basin.
Loss of genetic diversity as a signature of apricot domestication and diffusion into the Mediterranean Basin.
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DOI：
10.1186/1471-2229-12-49
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发表时间：
2012-04-17
影响因子：
5.3
通讯作者：
Khadari B
中科院分区：
文献类型：
--
作者：
Bourguiba H;Audergon JM;Krichen L;Trifi-Farah N;Mamouni A;Trabelsi S;D'Onofrio C;Asma BM;Santoni S;Khadari B
由于瓶颈效应造成的遗传漂变，驯化通常意味着作物物种相对于其野生祖先的多样性丧失。与橄榄和葡萄等地中海本土水果品种相比，引入地中海地区的水果品种（例如杏子（Prunus armeniaca L.））预计遗传多样性的丧失更为严重，杏子可能主要在中国驯化。通过比较几个地中海地区杏基因库的遗传多样性，我们研究了与杏选择和扩散到地中海盆地相关的遗传多样性丧失。根据杏子的地理起源并使用基因型的贝叶斯聚类，将地中海杏子（207个基因型）分为三个主要基因库：“伊朗-高加索”、“北地中海盆地”和“南地中海盆地”。在使用的 25 个微卫星标记中，只有一个显示出与中性状态下预期频率的偏差。基于中性假设，使用所有 SSR 位点和仅 24 个 SSR 位点在三个主要簇中的每一个中都获得了类似的遗传多样性参数。根据等位基因丰富度和私人等位基因丰富度的评估，“伊朗高加索人”基因库（被认为是地中海盆地北部和西南部多样化的次要中心）揭示了遗传多样性的显着丧失。在将“北地中海盆地”和“南地中海盆地”的基因库与第二多样化中心进行比较时，特别检测到了相当大比例的共享等位基因。通过地中海杏材料中的微卫星标记检测到明显的驯化瓶颈，描绘了“伊朗-高加索”基因库的两条扩散路线的全球图像：北地中海和西南地中海。这项研究产生了遗传见解，将有助于地中海杏种质的管理以及与适应性性状相关的遗传选择计划。
Domestication generally implies a loss of diversity in crop species relative to their wild ancestors because of genetic drift through bottleneck effects. Compared to native Mediterranean fruit species like olive and grape, the loss of genetic diversity is expected to be more substantial for fruit species introduced into Mediterranean areas such as apricot (Prunus armeniaca L.), which was probably primarily domesticated in China. By comparing genetic diversity among regional apricot gene pools in several Mediterranean areas, we investigated the loss of genetic diversity associated with apricot selection and diffusion into the Mediterranean Basin. According to the geographic origin of apricots and using Bayesian clustering of genotypes, Mediterranean apricot (207 genotypes) was structured into three main gene pools: ‘Irano-Caucasian’, ‘North Mediterranean Basin’ and ‘South Mediterranean Basin’. Among the 25 microsatellite markers used, only one displayed deviations from the frequencies expected under neutrality. Similar genetic diversity parameters were obtained within each of the three main clusters using both all SSR loci and only 24 SSR loci based on the assumption of neutrality. A significant loss of genetic diversity, as assessed by the allelic richness and private allelic richness, was revealed from the ‘Irano-Caucasian’ gene pool, considered as a secondary centre of diversification, to the northern and southwestern Mediterranean Basin. A substantial proportion of shared alleles was specifically detected when comparing gene pools from the ‘North Mediterranean Basin’ and ‘South Mediterranean Basin’ to the secondary centre of diversification. A marked domestication bottleneck was detected with microsatellite markers in the Mediterranean apricot material, depicting a global image of two diffusion routes from the ‘Irano-Caucasian’ gene pool: North Mediterranean and Southwest Mediterranean. This study generated genetic insight that will be useful for management of Mediterranean apricot germplasm as well as genetic selection programs related to adaptive traits.
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影响因子：
1.5
作者：
Bourguiba, Hedia;Khadari, Bouchaib;Audergon, Jean-Marc
通讯作者：
Audergon, Jean-Marc
影响因子：
2
作者：
Aranzana, MJ;Garcia-Mas, J;Arús, P
通讯作者：
Arús, P
影响因子：
2
作者：
Gharghani, A.;Zamani, Z.;Gardiner, S. E.
通讯作者：
Gardiner, S. E.
影响因子：
5.3
作者：
Besnard G;Hernández P;Khadari B;Dorado G;Savolainen V
通讯作者：
Savolainen V
影响因子：
3.8
作者：
Excoffier, L.;Hofer, T.;Foll, M.
通讯作者：
Foll, M.