Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells.
Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells.
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DOI：
10.1371/journal.pone.0078631
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Costa J
中科院分区：
文献类型：
--
作者：
Escrevente C;Grammel N;Kandzia S;Zeiser J;Tranfield EM;Conradt HS;Costa J
外泌体由包括肿瘤细胞和神经元在内的几种人类细胞分泌的囊泡组成，它们存在于几种生物液体中。外泌体具有特征性的蛋白质和脂质组成，然而，关于糖蛋白组成和糖基化的结果很少。本研究通过凝集素印迹、2-氨基苯甲酰胺标记聚糖的NP-HPLC分析和质谱分析，研究了卵巢癌SKOV3细胞外泌体的蛋白糖基化。外泌体中含有丰富的唾液糖蛋白，经肽质量指纹图谱和免疫印迹鉴定为半乳糖凝集素-3结合蛋白（LGALS3BP）。外泌体主要含有二链、三链和四链的复杂聚糖，有或没有近端病灶，也含有高甘露糖聚糖。还检测到含有分二n -乙酰氨基葡萄糖的双触角聚糖。本研究提供了卵巢癌细胞外泌体糖蛋白和n -聚糖组成的详细信息，为进一步探索聚糖在外泌体生物学中的功能作用开辟了新的视角。
Exosomes consist of vesicles that are secreted by several human cells, including tumor cells and neurons, and they are found in several biological fluids. Exosomes have characteristic protein and lipid composition, however, the results concerning glycoprotein composition and glycosylation are scarce. Here, protein glycosylation of exosomes from ovarian carcinoma SKOV3 cells has been studied by lectin blotting, NP-HPLC analysis of 2-aminobenzamide labeled glycans and mass spectrometry. An abundant sialoglycoprotein was found enriched in exosomes and it was identified by peptide mass fingerprinting and immunoblot as the galectin-3-binding protein (LGALS3BP). Exosomes were found to contain predominantly complex glycans of the di-, tri-, and tetraantennary type with or without proximal fucose and also high mannose glycans. Diantennary glycans containing bisecting N-acetylglucosamine were also detected. This work provides detailed information about glycoprotein and N-glycan composition of exosomes from ovarian cancer cells, furthermore it opens novel perspectives to further explore the functional role of glycans in the biology of exosomes.
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影响因子：
3.4
作者：
Abbott KL;Lim JM;Wells L;Benigno BB;McDonald JF;Pierce M
通讯作者：
Pierce M
影响因子：
3
作者：
Grassadonia, A;Tinari, N;Iacobelli, S
通讯作者：
Iacobelli, S
影响因子：
16
作者：
Bobrie A;Colombo M;Krumeich S;Raposo G;Théry C
通讯作者：
Théry C
影响因子：
14.9
作者：
Mathivanan S;Fahner CJ;Reid GE;Simpson RJ
通讯作者：
Simpson RJ
DOI：
10.1074/mcp.m111.011601
发表时间：
2012-09
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Balog CI;Stavenhagen K;Fung WL;Koeleman CA;McDonnell LA;Verhoeven A;Mesker WE;Tollenaar RA;Deelder AM;Wuhrer M
通讯作者：
Wuhrer M