Steady-state cross-correlations for live two-colour super-resolution localization data sets.
Steady-state cross-correlations for live two-colour super-resolution localization data sets.
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DOI:
10.1038/ncomms8347
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发表时间:
2015-06-12
影响因子:
16.6
通讯作者:
Veatch SL
中科院分区:
文献类型:
--
作者:
Stone MB;Veatch SL
Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation. Quantitative live cell super-resolution microscopy is currently limited by the time it takes to acquire a well sampled image. Here Stone and Veatch develop a cross-correlation analysis that does not rely on image reconstruction and apply this method to quantify the co-distribution of Lyn kinase and the B-cell receptor during antigen stimulation.
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影响因子:
48
作者:
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
通讯作者:
Zhuang, Xiaowei
DOI:
10.1083/jcb.200503110
发表时间:
2005-11-07
期刊:
The Journal of cell biology
影响因子:
--
作者:
Larson DR;Gosse JA;Holowka DA;Baird BA;Webb WW
通讯作者:
Webb WW
影响因子:
48
作者:
Sengupta, Prabuddha;Jovanovic-Talisman, Tijana;Skoko, Dunja;Renz, Malte;Veatch, Sarah L.;Lippincott-Schwartz, Jennifer
通讯作者:
Lippincott-Schwartz, Jennifer
DOI:
10.1073/pnas.1222097110
发表时间:
2013-07-23
影响因子:
11.1
作者:
Di Rienzo, Carmine;Gratton, Enrico;Cardarelli, Francesco
通讯作者:
Cardarelli, Francesco
影响因子:
3.4
作者:
Sezgin, Erdinc;Levental, Ilya;Schwille, Petra
通讯作者:
Schwille, Petra