Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage.
Analysis of the transcriptome of the protozoan Theileria parva using MPSS reveals that the majority of genes are transcriptionally active in the schizont stage.
复制标题
使用MPSS对原生动物Theileria parva的转录组分析表明，大多数基因在Schizont阶段具有转录活性。
DOI：
10.1093/nar/gki818
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发表时间：
2005
影响因子：
14.9
通讯作者：
de Villiers, EP
中科院分区：
文献类型：
--
作者：
Bishop, R;Shah, T;Pelle, R;Hoyle, D;Pearson, T;Haines, L;Brass, A;Hulme, H;Graham, SP;Taracha, ELN;Kanga, S;Lu, C;Hass, B;Wortman, J;White, O;Gardner, MJ;Nene, V;de Villiers, EP
大规模平行签名测序（MPSS）被用来分析细胞内的原生动物小泰勒虫的转录组。在总共1 095 000个中，从小锥虫中产生了代表4371个不同标签的20 bp序列。在73%的潜在可检测的预测基因中鉴定出可再现的签名，并且83%在至少一个MPSS循环中具有签名。在405个表达基因上检测到预测的前导肽。签名的数量范围为每百万（t. p.m.）4- 52256份转录本。罕见的成绩单（<50吨/分）从36%的基因中检测到。序列特征近似于对数正态分布，如在微阵列中。转录本广泛分布在整个基因组，虽然只有47%的138端粒相关的开放阅读框架表现出的签名。反义签名占总数的13.8%，与疟原虫相当。85个预测基因的反义签名缺乏一个有义签名。反义转录本从同源体cDNA独立扩增并通过测序验证。牛CD 8 T细胞识别的7个编码嗜酸性粒细胞抗原的基因的MPSS转录本每百万份变化1000倍。根据MPSS和蛋白质组学，对于非常高表达的热休克蛋白，转录和蛋白质表达之间是一致的。数据表明，大多数蛋白质编码基因的基线转录水平较低。
Massively parallel signature sequencing (MPSS) was used to analyze the transcriptome of the intracellular protozoan Theileria parva. In total 1 095 000, 20 bp sequences representing 4371 different signatures were generated from T.parva schizonts. Reproducible signatures were identified within 73% of potentially detectable predicted genes and 83% had signatures in at least one MPSS cycle. A predicted leader peptide was detected on 405 expressed genes. The quantitative range of signatures was 4–52 256 transcripts per million (t.p.m.). Rare transcripts (<50 t.p.m.) were detected from 36% of genes. Sequence signatures approximated a lognormal distribution, as in microarray. Transcripts were widely distributed throughout the genome, although only 47% of 138 telomere-associated open reading frames exhibited signatures. Antisense signatures comprised 13.8% of the total, comparable with Plasmodium. Eighty five predicted genes with antisense signatures lacked a sense signature. Antisense transcripts were independently amplified from schizont cDNA and verified by sequencing. The MPSS transcripts per million for seven genes encoding schizont antigens recognized by bovine CD8 T cells varied 1000-fold. There was concordance between transcription and protein expression for heat shock proteins that were very highly expressed according to MPSS and proteomics. The data suggests a low level of baseline transcription from the majority of protein-coding genes.
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影响因子：
4.6
作者：
Mecham, BH;Wetmore, DZ;Mariani, TJ
通讯作者：
Mariani, TJ
影响因子：
9.8
作者：
Bozdech, Zbynek;Llinas, Manuel;Pulliam, Brian Lee;Wong, Edith D;Zhu, Jingchun;DeRisi, Joseph L
通讯作者：
DeRisi, Joseph L
影响因子：
64.8
作者：
BROWN, CGD;STAGG, DA;PAYNE, RC
通讯作者：
PAYNE, RC
影响因子：
46.9
作者：
Meyers, BC;Vu, TH;Haudenschild, CD
通讯作者：
Haudenschild, CD
影响因子：
14.9
作者：
Mewes, HW;Frishman, D;Weil, B
通讯作者：
Weil, B