Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)
Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)
复制标题
激光捕获的生殖细胞的转录组分析和 17α-甲基睾酮诱导橙斑石斑鱼（Epinephelus coioides）精子发生过程中 zbtb40 的功能表征
DOI：
10.1186/s12864-020-6477-4
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发表时间：
2019-12
期刊：
影响因子：
4.4
通讯作者：
Xiaochun Liu
中科院分区：
文献类型：
--
作者：
Xi Wu;Yang;Chaoyue Zhong;Yin Guo;Shuisheng Li;Haoran Lin;Xiaochun Liu
背景
Background
Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the regulatory mechanism of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells isolated from orange-spotted grouper was performed to explore the molecular mechanism underlying spermatogenesis.
Results
In this study, the orange-spotted grouper was induced to change sex from female to male by 17alpha-methyltestosterone (MT) implantation. During the spermatogenesis, male germ cells (spermatogonia, spermatocytes, spermatids, and spermatozoa) were isolated by laser capture microdissection. Transcriptomic analysis for the isolated cells was performed. A total of 244,984,338 clean reads were generated from four cDNA libraries. Real-time PCR results of 13 genes related to sex differentiation and hormone metabolism indicated that transcriptome data are reliable. RNA-seq data showed that the female-related genes and genes involved in hormone metabolism were highly expressed in spermatogonia and spermatozoa, suggesting that these genes participate in the spermatogenesis. Interestingly, the expression of zbtb family genes showed significantly changes in the RNA-seq data, and their expression patterns were further examined during spermatogenesis. The analysis of cellular localization of Eczbtb40 and the co-localization of Eczbtb40 and Eccyp17a1 in different gonadal stages suggested that Eczbtb40 might interact with Eccyp17a1 during spermatogenesis.
Conclusions
Our study, for the first time, investigated the transcriptome of the male germ cells from orange-spotted grouper, and identified functional genes, GO terms, and KEGG pathways involved in spermatogenesis. Furthermore, Eczbtb40 was first characterized and its role during spermatogenesis was predicted. These data will contribute to future studies on the molecular mechanism of spermatogenesis in teleosts.
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影响因子：
20.3
作者：
J. F. Couse;K. Korach
通讯作者：
J. F. Couse;K. Korach
影响因子：
3.7
作者：
Li GL;Qian H
通讯作者：
Qian H
DOI：
10.1261/rna.067090.118
发表时间：
2018-12
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
He WX;Wu M;Liu Z;Li Z;Wang Y;Zhou J;Yu P;Zhang XJ;Zhou L;Gui JF
通讯作者：
Gui JF
甲基睾酮摄食和甲基睾酮戒断引起的雌性橙斑石斑鱼性别变化的分子调控
影响因子：
3.6
作者：
Wang Qing;Liu Yun;Peng Cheng;Wang Xiang;Xiao Ling;Wang Dengdong;Chen Jiaxing;Zhang Haifa;Zhao Huihong;Li Shuisheng;Zhang Yong;Lin Haoran
通讯作者：
Lin Haoran
DOI：
10.1111/rda.12960
发表时间：
2017-03
期刊：
Reproduction in domestic animals = Zuchthygiene
影响因子：
--
作者：
Keqiong Tang;Lei Wang;Yaping Jin;Wucai Yang;Liguo Yang
通讯作者：
Keqiong Tang;Lei Wang;Yaping Jin;Wucai Yang;Liguo Yang