Changes in expression of oncogenes and endogenous retroviral-like sequences during colon carcinogenesis.
Changes in expression of oncogenes and endogenous retroviral-like sequences during colon carcinogenesis.
复制标题
结肠癌发生过程中癌基因和内源性逆转录病毒样序列表达的变化。
作者：
J. Guillem;L. Hsieh;K. O'toole;K. Forde;P. Logerfo;I. Weinstein
通过分析两个原癌基因(c-myc和c-H-ras)、两个内源性逆转录病毒相关DNA序列[大鼠白血病病毒(RaLV)和30S序列]以及两个细胞周期相关基因(β-肌动蛋白和鸟氨酸脱羧酶)在偶氮甲烷诱导和高脂促进的结肠癌发生过程中的相应RNA水平，研究它们在实验性结肠癌发生中的可能作用。F-344雄性大鼠接受三次皮下注射。注射偶氮甲烷(15 mg/kg)或生理盐水，然后再分为高脂饮食组和低脂饮食组。在随后的连续处死过程中，取正常结肠粘膜、腺瘤和癌组织进行组织学和RNA提取。用合适的32P标记的DNA探针对71份RNA样品进行了Northern印迹杂交分析。C-myc、RaLV和30S RNAs在包括腺瘤和浸润性癌在内的所有结肠肿瘤中的表达均显著增加。RaLV和c-myc RNA在肿瘤旁扁平的正常粘膜和对照组大鼠的粘膜中未见表达或表达水平很低。一些结肠肿瘤还显示c-H-ras、鸟氨酸脱羧酶和β-肌动蛋白RNA水平升高，但这些发现没有c-myc、RaLV和30S RNA那么显著，也更具变异性。这些结果表明，c-myc原癌基因和内源性逆转录病毒样序列(RaLV)和30S的表达增加是该模型系统中结肠癌发生的标志。
The possible roles in experimental colon carcinogenesis of two protooncogenes (c-myc and c-H-ras), two endogenous retrovirus-related DNA sequences [rat leukemia virus (RaLV) and the 30S sequence], and two cell cycle related genes (beta-actin and ornithine decarboxylase) were studied by analyzing the levels of their corresponding RNAs during the course of azoxymethane induced and high fat promoted colon carcinogenesis. F-344 male rats received three s.c. injections of azoxymethane (15 mg/kg) or normal saline and were then subdivided into high or low fat diet groups. During subsequent serial sacrifices normal colon mucosa, adenomas, and carcinomas were harvested for histology and RNA extraction. Seventy-one RNA samples were analyzed by the Northern blot hybridization procedure using the appropriate 32P-labeled DNA probes. A marked increase in the abundance of c-myc, RaLV, and 30S RNAs were seen in all of the colon tumors, including adenomas and invasive carcinomas. No or a very low level of expression of RaLV and c-myc RNA was found in the flat grossly normal mucosa adjacent to the tumors and in the mucosa of the control rats. Some of the colon tumors also displayed increased levels of c-H-ras, ornithine decarboxylase and beta-actin RNAs but these findings were less striking and more variable than those seen with c-myc, RaLV, and 30S RNAs. These results suggest that increased expression of the c-myc protooncogene and of the endogenous retrovirus-like sequences (RaLV) and 30S are hallmarks of colon carcinogenesis in this model system.
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二乙基亚硝胺诱导的大鼠肝肿瘤中逆转录病毒序列和癌基因的表达。
影响因子：
11.2
作者：
Hsieh,LL;Hsiao,WL;Peraino,C;Maronpot,RR;Weinstein,IB
通讯作者：
Weinstein,IB
结肠癌和腺瘤性息肉中的原癌基因异常。
影响因子：
29.4
作者：
Meltzer,SJ;Ahnen,DJ;Battifora,H;Yokota,J;Cline,MJ
通讯作者：
Cline,MJ
DOI：
10.20772/cancersci1959.75.1_29
发表时间：
1984
期刊：
Gan
影响因子：
--
作者：
S. Takano;M. Akagi;G. Bryan
通讯作者：
S. Takano;M. Akagi;G. Bryan
Ha-ras 表达升高是 SENCAR 小鼠两阶段皮肤癌发生的早期事件。
影响因子：
4.7
作者：
Pelling,JC;Ernst,SM;Strawhecker,JM;Johnson,JA;Nairn,RS;Slaga,TJ
通讯作者：
Slaga,TJ
影响因子：
56.9
作者：
SLAMON, DJ;DEKERNION, JB;CLINE, MJ
通讯作者：
CLINE, MJ