The Characterization of a Novel Virus Discovered in the Yeast Pichia membranifaciens.
The Characterization of a Novel Virus Discovered in the Yeast Pichia membranifaciens.
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DOI：
10.3390/v14030594
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发表时间：
2022-03-13
期刊：
影响因子：
--
通讯作者：
Rowley PA
中科院分区：
文献类型：
--
作者：
Lee MD;Creagh JW;Fredericks LR;Crabtree AM;Patel JS;Rowley PA
真菌病毒广泛分布于真菌中，包括酵母亚门的酵母。本文报道了从膜状毕赤酵母中分离到的第一个双链RNA病毒。这一新病毒被命名为膜面毕赤酵母病毒L-A(PMV-L-A)，是Totiviridae的成员。PMV-L-A全长4579bp，二级结构与侵染酵母菌的杆状病毒的包装、复制和移码信号相似。发现PMV-L-A属于I-A群中的一个单系群，这意味着从毕赤酵母和酵母科酵母中分离到的分支病毒具有共同的祖先。能量最小化的PMV-L-A Gag蛋白的AlphaFold2分子模型揭示了与酿酒酵母病毒L-A(SCV-L-A)Gag蛋白的结构保守性。预测的PMV-L-A POL和其他同源物的三级结构提供了一种可能的机制，因为它与哺乳动物双链RNA病毒依赖RNA的RNA聚合酶结构相似，因此可能是TotiVirus复制的机制。从酵母中获得对TotiVirus的结构、功能和进化的洞察是至关重要的，因为它们在动物疾病中的新作用以及它们与哺乳动物病毒的相似之处。
Mycoviruses are widely distributed across fungi, including the yeasts of the Saccharomycotina subphylum. This manuscript reports the first double-stranded RNA (dsRNA) virus isolated from Pichia membranifaciens. This novel virus has been named Pichia membranifaciens virus L-A (PmV-L-A) and is a member of the Totiviridae. PmV-L-A is 4579 bp in length, with RNA secondary structures similar to the packaging, replication, and frameshift signals of totiviruses that infect Saccharomycotina yeasts. PmV-L-A was found to be part of a monophyletic group within the I-A totiviruses, implying a shared ancestry between mycoviruses isolated from the Pichiaceae and Saccharomycetaceae yeasts. Energy-minimized AlphaFold2 molecular models of the PmV-L-A Gag protein revealed structural conservation with the Gag protein of Saccharomyces cerevisiae virus L-A (ScV-L-A). The predicted tertiary structure of the PmV-L-A Pol and other homologs provided a possible mechanism for totivirus RNA replication due to structural similarities with the RNA-dependent RNA polymerases of mammalian dsRNA viruses. Insights into the structure, function, and evolution of totiviruses gained from yeasts are essential because of their emerging role in animal disease and their parallels with mammalian viruses.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
4.5
作者：
Fan, Q;Tian, SP
通讯作者：
Tian, SP
影响因子：
3.2
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Kubota, Kenji;Ng, James C. K.
通讯作者：
Ng, James C. K.
影响因子：
4.2
作者：
Belda I;Ruiz J;Alonso A;Marquina D;Santos A
通讯作者：
Santos A
影响因子：
2.6
作者：
Masih, EI;Paul, B
通讯作者：
Paul, B