Actin grips: circular actin-rich cytoskeletal structures that mediate the wrapping of polymeric microfibers by endothelial cells.
Actin grips: circular actin-rich cytoskeletal structures that mediate the wrapping of polymeric microfibers by endothelial cells.
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DOI：
10.1016/j.biomaterials.2015.02.034
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发表时间：
2015-06
期刊：
影响因子：
14
通讯作者：
Moldovan, Nicanor I.
中科院分区：
文献类型：
--
作者：
Jones, Desiree;Park, DoYoung;Anghelina, Mirela;Pecot, Thierry;Machiraju, Raghu;Xue, Ruipeng;Lannutti, John J.;Thomas, Jessica;Cole, Sara L.;Moldovan, Leni;Moldovan, Nicanor I.
与平坦培养表面相比，内皮谱系细胞与三维基质的相互作用的研究要少得多。我们研究了成熟内皮细胞 (EC) 和分化程度较低的 EC 集落形成细胞与直径在 5-20 μm 范围内的聚己内酯 (PCL) 纤维（“支架微纤维”，SMF）的体外附着情况。我们发现，尽管两种细胞类型对 PCL 的固有粘附性较差，但经过长期培养后，两种细胞类型都完全包裹了 SMF，从而获得了圆柱形形态。在这个系统中，两种 EC 类型都旺盛生长了一周多，并且变得越来越分化，如多重基因表达所示。使用定制软件对多光子共焦显微镜图像进行三维重建表明，丝状 (F) 肌动蛋白束在 SMF 周围呈现出明显的环状组织。与经典的含 F-肌动蛋白的应力纤维不同，这些环与粘着斑或中间丝无关。我们还证明，与这些圆形细胞骨架结构相邻的质膜边界紧密但动态地与 SMF 相对应，因此我们建议将它们称为“肌动蛋白夹”。总之，我们描述了一种特殊形式的 F-肌动蛋白组装，其与响应生物材料的细胞骨架组织、体外和体内内皮特异性细胞行为以及组织工程相关。
Interaction of endothelial-lineage cells with three-dimensional substrates was much less studied than that with flat culture surfaces. We investigated the in vitro attachment of both mature endothelial cells (ECs) and of less differentiated EC colony-forming cells to poly-e-capro-lactone (PCL) fibers with diameters in 5–20 μm range (‘scaffold microfibers’, SMFs). We found that notwithstanding the poor intrinsic adhesiveness to PCL, both cell types completely wrapped the SMFs after long-term cultivation, thus attaining a cylindrical morphology. In this system, both EC types grew vigorously for more than a week and became increasingly more differentiated, as shown by multiplexed gene expression. Three-dimensional reconstructions from multiphoton confocal microscopy images using custom software showed that the filamentous (F) actin bundles took a conspicuous ring-like organization around the SMFs. Unlike the classical F-actin-containing stress fibers, these rings were not associated with either focal adhesions or intermediate filaments. We also demonstrated that plasma membrane boundaries adjacent to these circular cytoskeletal structures were tightly yet dynamically apposed to the SMFs, for which reason we suggest to call them ‘actin grips’. In conclusion, we describe a particular form of F-actin assembly with relevance for cytoskeletal organization in response to biomaterials, for endothelial-specific cell behavior in vitro and in vivo, and for tissue engineering.
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影响因子：
3.7
作者：
DUNN, GA;HEATH, JP
通讯作者：
HEATH, JP
DOI：
10.1073/pnas.0504271103
发表时间：
2006-01-03
影响因子：
11.1
作者：
Gavrilin, MA;Bouakl, IJ;Wewers, MD
通讯作者：
Wewers, MD
影响因子：
4
作者：
Kovac, Bianca;Teo, Jessica L.;Vallenius, Tea
通讯作者：
Vallenius, Tea
DOI：
10.1002/jbm.b.31916
发表时间：
2012-02-01
影响因子：
3.4
作者：
Fu, Xin;Sammons, Rachel L.;Dong, Hanshan
通讯作者：
Dong, Hanshan
影响因子：
14
作者：
Guan, Jianjun;Wang, Feng;Li, Zhenqing;Chen, Joseph;Guo, Xiaolei;Liao, Jun;Moldovan, Nicanor I.
通讯作者：
Moldovan, Nicanor I.