Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222.
Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222.
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DOI：
10.1038/s41598-021-96559-2
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发表时间：
2021-08-26
影响因子：
4.6
通讯作者：
Roldán MD
中科院分区：
文献类型：
--
作者：
Olaya-Abril A;Hidalgo-Carrillo J;Luque-Almagro VM;Fuentes-Almagro C;Urbano FJ;Moreno-Vivián C;Richardson DJ;Roldán MD
反硝化作用是一个呼吸过程，通过它硝酸盐被还原成二氮。不完全的反硝化导致温室气体一氧化二氮的排放，这在酸性土壤中是增强的，与碱性土壤相比，酸性土壤显示出降低的反硝化速率和高的N2 O/N2比率。在这项工作中，pH值的影响，对土壤微生物Paracoccus reprodicans PD 1222的蛋白质组进行了分析，硝酸盐作为唯一的能源和氮源，在厌氧条件下，pH值为6.5至7.5。定量蛋白质组学分析表明，在pH 6.5的蛋白质组相比，在pH 7.2的参考蛋白质组时，观察到的蛋白质表示的最高差异。然而，细胞外pH的这种差异不足以产生细胞内pH的改变，细胞内pH维持在6.5 ± 0.1。反硝化和氮同化相关的几个辅因子，如钴胺素，核黄素，异蝶呤和烟酰胺的生物合成途径在pH 6.5的负面影响。此外，参与硝酸盐同化和反硝化的还原酶的肽表示在pH 6.5时减少。数据强调了pH对NosZ合成和细胞内铜含量的强烈负面影响，从而损害了活性NosZ组装，进而导致一氧化二氮排放量升高。
Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which display reduced denitrification rates and high N2O/N2 ratios compared to alkaline soils. In this work, impact of pH on the proteome of the soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with nitrate as sole energy and nitrogen source under anaerobic conditions at pH ranging from 6.5 to 7.5. Quantitative proteomic analysis revealed that the highest difference in protein representation was observed when the proteome at pH 6.5 was compared to the reference proteome at pH 7.2. However, this difference in the extracellular pH was not enough to produce modification of intracellular pH, which was maintained at 6.5 ± 0.1. The biosynthetic pathways of several cofactors relevant for denitrification and nitrogen assimilation like cobalamin, riboflavin, molybdopterin and nicotinamide were negatively affected at pH 6.5. In addition, peptide representation of reductases involved in nitrate assimilation and denitrification were reduced at pH 6.5. Data highlight the strong negative impact of pH on NosZ synthesis and intracellular copper content, thus impairing active NosZ assembly and, in turn, leading to elevated nitrous oxide emissions.
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影响因子：
3.2
作者：
HARMS, N;DEVRIES, GE;STOUTHAMER, AH
通讯作者：
STOUTHAMER, AH
DOI：
10.1098/rstb.2011.0321
发表时间：
2012-05-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Bakken LR;Bergaust L;Liu B;Frostegård A
通讯作者：
Frostegård A
DOI：
10.1042/bcj20170115
发表时间：
2017-05-10
期刊：
The Biochemical journal
影响因子：
--
作者：
Luque-Almagro VM;Manso I;Sullivan MJ;Rowley G;Ferguson SJ;Moreno-Vivián C;Richardson DJ;Gates AJ;Roldán MD
通讯作者：
Roldán MD
影响因子：
5.1
作者：
Bergaust, Linda;Shapleigh, James;Bakken, Lars
通讯作者：
Bakken, Lars
影响因子：
3.9
作者：
Luque-Almagro, Victor M.;Gates, Andrew J.;Dolores Roldan, M.
通讯作者：
Dolores Roldan, M.